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The humidity level matters during the desiccation of Norway spruce somatic embryos
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SYSNO ASEP 0562063 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title The humidity level matters during the desiccation of Norway spruce somatic embryos Author(s) Fischerová, Lucie (UEB-Q) RID, ORCID
Gemperlová, Lenka (UEB-Q) ORCID, RID
Cvikrová, Milena (UEB-Q) RID, ORCID
Matušíková, I. (SK)
Moravčíková, J. (SK)
Gerši, Z. (SI)
Malbeck, Jiří (UEB-Q) RID, ORCID
Kuderna, Jan (UEB-Q)
Pavlíčková, Jana (UEB-Q)
Motyka, Václav (UEB-Q) RID, ORCID
Eliášová, Kateřina (UEB-Q) RID, ORCID
Vondráková, Zuzana (UEB-Q) RID, ORCIDNumber of authors 12 Article number 968982 Source Title Frontiers in Plant Science. - : Frontiers Research Foundation - ISSN 1664-462X
Roč. 13, JUL 29 (2022)Number of pages 20 s. Language eng - English Country CH - Switzerland Keywords 3-glucanases ; chitinases ; desiccation ; germination ; Picea abies (L.) Karst ; polyamines ; somatic embryogenesis ; beta-1,3-glucanases OECD category Plant sciences, botany R&D Projects LTC17036 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support UEB-Q - RVO:61389030 UT WOS 000861301600001 EID SCOPUS 85135944570 DOI 10.3389/fpls.2022.968982 Annotation In Norway spruce, as in many other conifers, the germination capacity of somatic embryos is strongly influenced by the desiccation phase inserted after maturation. The intensity of drying during desiccation eminently affected the formation of emblings (i.e., seedlings developed from somatic embryos). Compared to non-desiccated embryos, the germination capacity of embryos desiccated at 100% relative humidity was about three times higher, but the reduction of relative humidity to 95 and 90% had a negative effect on the subsequent embryo development. The water loss observed in these embryos did not lead to an increase in lipid peroxidation, as shown by malondialdehyde levels. Another metabolic pathway in plants that mediates a response to abiotic stresses is directed toward the biosynthesis of polyamines (PAs). The activities of PA biosynthetic enzymes increased steadily in embryos during desiccation at 100% relative humidity, whereas they decreased at lower humidity. The total content of free PAs in the embryos gradually decreased throughout desiccation. The increase in free putrescine (Put) and perchloric acid-insoluble Put conjugates was observed in embryos desiccated at lower humidity. These changes were accompanied to some extent by the transcription of the genes for the PA biosynthesis enzymes. Desiccation at 100% relative humidity increased the activity of the cell wall-modifying enzymes β-1,3-glucanases and chitinases, the activities of these enzymes were also significantly suppressed at reduced humidity. The same pattern was observed in the transcription of some β-1,3-glucanase and chitinase genes. Desiccation treatments triggered metabolic processes that responded to water availability, suggesting an active response of the embryo to the reduction in humidity. A positive effect was demonstrated only for desiccation at high relative humidity. Some of the physiological characteristics described can be used as markers of inappropriate relative humidity during somatic embryo desiccation. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2023 Electronic address https://doi.org/10.3389/fpls.2022.968982
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