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Photosystem II antenna modules CP43 and CP47 do not form a stable 'no reaction centre complex' in the cyanobacterium Synechocystis sp. PCC 6803
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SYSNO ASEP 0561359 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Photosystem II antenna modules CP43 and CP47 do not form a stable 'no reaction centre complex' in the cyanobacterium Synechocystis sp. PCC 6803 Author(s) Bečková, Martina (MBU-M) RID
Sobotka, Roman (MBU-M) RID, ORCID
Komenda, Josef (MBU-M) RID, ORCIDSource Title Photosynthesis Research. - : Springer - ISSN 0166-8595
Roč. 152, č. 3 (2022), s. 363-371Number of pages 9 s. Language eng - English Country NL - Netherlands Keywords Photosystem II ; Photosynthesis ; cp43 ; cp47 ; No reaction centre complex Subject RIV EF - Botanics OECD category Plant sciences, botany R&D Projects GX19-29225X GA ČR - Czech Science Foundation (CSF) Method of publishing Open access Institutional support MBU-M - RVO:61388971 UT WOS 000741214600001 EID SCOPUS 85122690997 DOI 10.1007/s11120-022-00896-w Annotation The repair of photosystem II is a key mechanism that keeps the light reactions of oxygenic photosynthesis functional. During this process, the PSII central subunit D1 is replaced with a newly synthesized copy while the neighbouring CP43 antenna with adjacent small subunits (CP43 module) is transiently detached. When the D2 protein is also damaged, it is degraded together with D1 leaving both the CP43 module and the second PSII antenna module CP47 unassembled. In the cyanobacterium Synechocystis sp. PCC 6803, the released CP43 and CP47 modules have been recently suggested to form a so-called no reaction centre complex (NRC). However, the data supporting the presence of NRC can also be interpreted as a co-migration of CP43 and CP47 modules during electrophoresis and ultracentrifugation without forming a mutual complex. To address the existence of NRC, we analysed Synechocystis PSII mutants accumulating one or both unassembled antenna modules as well as Synechocystis wild-type cells stressed with high light. The obtained results were not compatible with the existence of a stable NRC since each unassembled module was present as a separate protein complex with a mutually similar electrophoretic mobility regardless of the presence of the second module. The non-existence of NRC was further supported by isolation of the His-tagged CP43 and CP47 modules from strains lacking either D1 or D2 and their migration patterns on native gels. Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2023 Electronic address https://link.springer.com/article/10.1007/s11120-022-00896-w
Number of the records: 1