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Age-related loss of axonal regeneration is reflected by the level of local translation
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SYSNO ASEP 0560416 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Age-related loss of axonal regeneration is reflected by the level of local translation Author(s) van Erp, S. (GB)
van Berkel, M. (NL)
Feenstra, E.M. (GB)
Sahoo, P. K. (US)
Wagstaff, L.J. (GB)
Twiss, J. L. (US)
Fawcett, James (UEM-P) ORCID
Eva, R. (GB)
Ffrench-Constant, Ch. (GB)Article number 113594 Source Title Experimental Neurology. - : Elsevier - ISSN 0014-4886
Roč. 339, may. (2021)Number of pages 12 s. Language eng - English Country US - United States Keywords axon regeneration ; human stem cells ; local translation ; proteomics ; in vitro live imaging ; axotomy OECD category Neurosciences (including psychophysiology Method of publishing Open access Institutional support UEM-P - RVO:68378041 UT WOS 000634819800002 EID SCOPUS 85100656511 DOI 10.1016/j.expneurol.2020.113594 Annotation Regeneration capacity is reduced as CNS axons mature. Using laser-mediated axotomy, proteomics and pummycin-based tagging of newly-synthesized proteins in a human embryonic stem cell-derived neuron culture system that allows isolation of axons from cell bodies, we show here that efficient regeneration in younger axons (d45 in culture) is associated with local axonal protein synthesis (local translation). Enhanced regeneration, promoted by co-culture with human glial precursor cells, is associated with increased axonal synthesis of proteins, including those constituting the translation machinery itself. Reduced regeneration, as occurs with the maturation of these axons by d65 in culture, correlates with reduced levels of axonal proteins involved in translation and an inability to respond by increased translation of regeneration promoting axonal mRNAs released from stress granules. Together, our results provide evidence that, as in development and in the PNS, local translation contributes to CNS axon regeneration. Workplace Institute of Experimental Medicine Contact Lenka Koželská, lenka.kozelska@iem.cas.cz, Tel.: 241 062 218, 296 442 218 Year of Publishing 2023 Electronic address https://www.sciencedirect.com/science/article/pii/S0014488620304258?via%3Dihub
Number of the records: 1