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Mitochondrial Contact Site and Cristae Organization System and F1FO-ATP Synthase Crosstalk Is a Fundamental Property of Mitochondrial Cristae (vol 6, e00327-21, 2021)
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SYSNO ASEP 0557503 Document Type O - Others R&D Document Type Others Title Mitochondrial Contact Site and Cristae Organization System and F1FO-ATP Synthase Crosstalk Is a Fundamental Property of Mitochondrial Cristae (vol 6, e00327-21, 2021) Author(s) Cadena, Lawrence Rudy (BC-A) RID, ORCID
Gahura, Ondřej (BC-A) RID, ORCID
Panicucci, Brian (BC-A)
Zíková, Alena (BC-A) RID, ORCID
Hashimi, Hassan (BC-A) RID, ORCIDNumber of authors 5 Year of issue 2022 Article number e00327-21 Source Title mSphere. - : American Society for Microbiology - ISSN 2379-5042
Roč. 6, č. 3 (2022)Number of pages 1 s. Language eng - English Country US - United States Keywords atp synthase ; organizing system ; f1f0-atp synthase Subject RIV EA - Cell Biology OECD category Cell biology R&D Projects GA20-23513S GA ČR - Czech Science Foundation (CSF) GA18-17529S GA ČR - Czech Science Foundation (CSF) EF16_019/0000759 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LM2015062 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GJ20-04150Y GA ČR - Czech Science Foundation (CSF) Institutional support BC-A - RVO:60077344 UT WOS 000789756000001 DOI 10.1128/msphere.00189-22 Annotation Mitochondrial cristae are polymorphic invaginations of the inner membrane that are the fabric of cellular respiration. Both the mitochondrial contact site and cristae organization system (MICOS) and the F1FO-ATP synthase are vital for sculpting cristae by opposing membrane-bending forces. While MICOS promotes negative curvature at crista junctions, dimeric F1FO-ATP synthase is crucial for positive curvature at crista rims. Crosstalk between these two complexes has been observed in baker's yeast, the model organism of the Opisthokonta supergroup. Here, we report that this property is con-served in Trypanosoma brucei, a member of the Discoba clade that separated from the Opisthokonta similar to 2 billion years ago. Specifically, one of the paralogs of the core MICOS subunit Mic10 interacts with dimeric F1FO-ATP synthase, whereas the other core Mic60 subunit has a counteractive effect on F1FO-ATP synthase oligomerization. This is evoca-tive of the nature of MICOS-F1FO-ATP synthase crosstalk in yeast, which is remarkable given the diversification that these two complexes have undergone during almost 2 eons of independent evolution. Furthermore, we identified a highly diverged, putative homolog of subunit e, which is essential for the stability of F1FO-ATP synthase dimers in yeast. Just like subunit e, it is preferentially associated with dimers and interacts with Mic10, and its silencing results in severe defects to cristae and the disintegration of F1FO-ATP synthase dimers. Our findings indicate that crosstalk between MICOS and dimeric F1FO-ATP synthase is a fundamental property impacting crista shape throughout eukaryotes. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2022 Electronic address https://journals.asm.org/doi/epub/10.1128/msphere.00189-22
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