Raw Goat's Milk, Fresh and Soft Cheeses as a Potential Source of Encephalitozoon cuniculi

Vecková, Tereza; Sak, Bohumil; Samková, E.; Holubová, Nikola; Kicia, M.; Zajaczkowska, Z.; Hlásková, Lenka; Květoňová, Dana; Kváč, Martin

doi:https://dx.doi.org/10.1089/fpd.2021.0017

Number of the records: 1

Raw Goat's Milk, Fresh and Soft Cheeses as a Potential Source of Encephalitozoon cuniculi

1.

SYSNO ASEP	0555214
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Raw Goat's Milk, Fresh and Soft Cheeses as a Potential Source of Encephalitozoon cuniculi
Author(s)	Vecková, Tereza (BC-A)_SAI Sak, Bohumil (BC-A)_{RID, ORCID} Samková, E. (CZ) Holubová, Nikola (BC-A)_{SAI, ORCID} Kicia, M. (PL) Zajaczkowska, Z. (PL) Hlásková, Lenka (BC-A)_SAI Květoňová, Dana (BC-A)_SAI Kváč, Martin (BC-A)_{RID, RID, ORCID}
Number of authors	9
Source Title	Foodborne pathogens and disease. - : Mary Ann Liebert - ISSN 1535-3141 Roč. 18, č. 9 (2021), s. 661-667
Number of pages	7 s.
Publication form	Online - E
Language	eng - English
Country	US - United States
Keywords	infection ; antibodies ; intestinalis ; microsporidia ; humans ; brain ; sheep ; cheese processing ; foodborne disease ; experimental infection ; infectivity ; coagulation
Subject RIV	FN - Epidemiology, Contagious Diseases ; Clinical Immunology
OECD category	Infectious Diseases
R&D Projects	GA20-10706S GA ČR - Czech Science Foundation (CSF)
Method of publishing	Limited access
Institutional support	BC-A - RVO:60077344
UT WOS	000649417300001
EID SCOPUS	85113291620
DOI	10.1089/fpd.2021.0017
Annotation	The aim of this work was to evaluate the effect of pasteurization and coagulation during goat cheese production on the infectivity of Encephalitozoon cuniculi spores for immunodeficient (SCID, CD4(-/-), and CD8(-/-)) and immunocompetent (BALB/c and C57BL/6) mice. Goat milk and fecal samples were screened for the presence and quantity of microsporidial DNA using molecular methods. Experimentally produced cheese from E. cuniculi-enriched goat milk or goat cheese purchased from retail producers was fed with experimental mice susceptible to E. cuniculi infection. The mice were sacrificed in the presumed acute phase of infection and samples of their tissues were subject to molecular detection of specific E. cuniculi DNA. Specific DNA of E. cuniculi genotype II was detected in feces and milk of three out of 99 goats kept on 6 farms in the Czech Republic. Under experimental conditions, spores of E. cuniculi genotype II remained viable in artificially enriched fresh cheese and were able to cause infection in laboratory mice. E. cuniculi genotype I and II DNA were detected in eight of the nine goat cheeses purchased from various producers/breeders in the Czech Republic and these cheeses were able to develop infection in both immunodeficient and immunocompetent mice. The results of these experiments showed that spores of E. cuniculi genotype I and II are able to remain viable after cheese processing and thus fresh and soft cheeses should be considered a potential source of microsporidia.
Workplace	Biology Centre (since 2006)
Contact	Dana Hypšová, eje@eje.cz, Tel.: 387 775 214
Year of Publishing	2022
Electronic address	https://www.liebertpub.com/doi/10.1089/fpd.2021.0017

Number of the records: 1