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Biological autoluminescence for assessing oxidative processes in yeast cell cultures
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SYSNO ASEP 0553145 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Biological autoluminescence for assessing oxidative processes in yeast cell cultures Author(s) Vahalová, Petra (URE-Y)
Červinková, Kateřina (URE-Y)
Cifra, Michal (URE-Y) RID, ORCID, SAINumber of authors 3 Article number 10852 Source Title Scientific Reports. - : Nature Publishing Group - ISSN 2045-2322
Roč. 11, č. 1 (2021)Number of pages 10 s. Publication form Print - P Language eng - English Country GB - United Kingdom Keywords Hydrogen-Ion Concentration ; Reactive oxygen metabolite ; Luminescent Measurements ; Oxidation-Reduction ; Saccharomyces cerevisiae Subject RIV JA - Electronics ; Optoelectronics, Electrical Engineering OECD category Electrical and electronic engineering R&D Projects GX20-06873X GA ČR - Czech Science Foundation (CSF) Method of publishing Open access Institutional support URE-Y - RVO:67985882 UT WOS 000659129600022 EID SCOPUS 85106908746 DOI 10.1038/s41598-021-89753-9 Annotation Nowadays, modern medicine is looking for new, more gentle, and more efficient diagnostic methods. A pathological state of an organism is often closely connected with increased amount of reactive oxygen species. They can react with biomolecules and subsequent reactions can lead to very low endogenous light emission (biological autoluminescence-BAL). This phenomenon can be potentially used as a non-invasive and low-operational-cost tool for monitoring oxidative stress during diseases. To contribute to the understanding of the parameters affecting BAL, we analyzed the BAL from yeast Saccharomyces cerevisiae as a representative eukaryotic organism. The relationship between the BAL intensity and the amount of reactive oxygen species that originates as a result of the Fenton reaction as well as correlation between spontaneous BAL and selected physical and chemical parameters (pH, oxygen partial pressure, and cell concentration) during cell growth were established. Our results contribute to real-time non-invasive methodologies for monitoring oxidative processes in biomedicine and biotechnology Workplace Institute of Radio Engineering and Electronics Contact Petr Vacek, vacek@ufe.cz, Tel.: 266 773 413, 266 773 438, 266 773 488 Year of Publishing 2022 Electronic address https://doi.org/10.1038/s41598-021-89753-9
Number of the records: 1