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LARP6C orchestrates posttranscriptional reprogramming of gene expression during hydration to promote pollen tube guidance
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SYSNO ASEP 0549798 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title LARP6C orchestrates posttranscriptional reprogramming of gene expression during hydration to promote pollen tube guidance Author(s) Billey, E. (FR)
Hafidh, Said (UEB-Q) RID, ORCID
Cruz-Gallardo, I. (GB)
Litholdo, C. G. (FR)
Jean, V. (FR)
Carpentier, M. C. (FR)
Picart, C. (FR)
Kumar, Vinod (UEB-Q) ORCID
Kulichová, Katarína (UEB-Q) ORCID
Maréchal, V. (FR)
Honys, David (UEB-Q) RID, ORCID
Conte, M. R. (GB)
Deragon, J. M. (FR)
Bousquet-Antonelli, C. (FR)Number of authors 14 Source Title Plant Cell. - : Oxford University Press - ISSN 1040-4651
Roč. 33, č. 8 (2021), s. 2637-2661Number of pages 25 s. Language eng - English Country US - United States Keywords MESSENGER-RNA LOCALIZATION ; ARABIDOPSIS-THALIANA ; TRANSCRIPTION FACTOR OECD category Biochemistry and molecular biology R&D Projects GA17-23203S GA ČR - Czech Science Foundation (CSF) GA18-02448S GA ČR - Czech Science Foundation (CSF) EF16_019/0000738 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support UEB-Q - RVO:61389030 UT WOS 000696248800014 EID SCOPUS 85114190592 DOI 10.1093/plcell/koab131 Annotation Increasing evidence suggests that posttranscriptional regulation is a key player in the transition between mature pollen and the progamic phase (from pollination to fertilization). Nonetheless, the actors in this messenger RNA (mRNA)-based gene expression reprogramming are poorly understood. We demonstrate that the evolutionarily conserved RNA-binding protein LARP6C is necessary for the transition from dry pollen to pollen tubes and the guided growth of pollen tubes towards the ovule in Arabidopsis thaliana. In dry pollen, LARP6C binds to transcripts encoding proteins that function in lipid synthesis and homeostasis, vesicular trafficking, and polarized cell growth. LARP6C also forms cytoplasmic granules that contain the poly(A) binding protein and possibly represent storage sites for translationally silent mRNAs. In pollen tubes, the loss of LARP6C negatively affects the quantities and distribution of storage lipids, as well as vesicular trafficking. In Nicotiana benthamiana leaf cells and in planta, analysis of reporter mRNAs designed from the LARP6C target MGD2 provided evidence that LARP6C can shift from a repressor to an activator of translation when the pollen grain enters the progamic phase. We propose that LARP6C orchestrates the timely posttranscriptional regulation of a subset of mRNAs in pollen during the transition from the quiescent to active state and along the progamic phase to promote male fertilization in plants. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2022 Electronic address http://doi.org/10.1093/plcell/koab131
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