0548345 - BC 2022 RIV IE eng J - Journal Article
Vráblová, M. - Marková, D. - Vrábl, D. - Koutník, I. - Sokolová, B. - Hronková, Marie
Surface plasmon resonance: An innovative method for studying water permeability of plant cuticles.
Plant Science. Roč. 310, Sep 2021 (2021), č. článku 110978. ISSN 0168-9452. E-ISSN 1873-2259
R&D Projects: GA ČR(CZ) GA18-14704S
Institutional support: RVO:60077344
Keywords : Surface plasmon resonance * Water permeability * Conductance * Cuticle * Wax * Ficus elastica
OECD category: Plant sciences, botany
Impact factor: 5.363, year: 2021
Method of publishing: Open access
https://www.sciencedirect.com/science/article/pii/S0168945221001746?via%3Dihub

The cuticle forms an effective barrier protecting plants from water loss. Its permeability to water and other compounds significantly differs between species, types of cuticle (stomatous, astomatous), and can be affected by a wide variety of ambient conditions. Enzymatic isolation of the leaf cuticle allows obtaining intact cuticles for permeability measurements. However, the most available gravimetric method, which is used for the assessment of water permeability of isolated cuticles, requires a relatively large area of the cuticle and does not allow the determination of membrane heterogeneity. We propose a new method for the determination of water permeance based on an on-line detection of water flux from a liquid phase to the atmosphere through isolated leaf cuticles in semi-flow chambers. This approach is new in using the phenomenon of surface plasmon resonance for the detection of the liquid phase refractive index affected by water vapor. Isolated cuticles of the leaves of Ficus elastica and an artificial polyethersulfone membrane were used for method evaluation. The composition of cuticular wax and its influence on cuticular permeability was also studied. It has been confirmed that the application of the surface plasmon resonance principle can be used for the assessment of leaf cuticle water permeability and heterogeneity.
Permanent Link: http://hdl.handle.net/11104/0329080