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Mitochondrial Retrograde Signaling Contributes to Metabolic Differentiation in Yeast Colonies
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SYSNO ASEP 0545739 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Mitochondrial Retrograde Signaling Contributes to Metabolic Differentiation in Yeast Colonies Author(s) Plocek, V. (CZ)
Fadrhonc, K. (CZ)
Maršíková, J. (CZ)
Váchová, Libuše (MBU-M) RID, ORCID
Pokorná, Alexandra (MBU-M)
Hlaváček, Otakar (MBU-M) RID, ORCID
Wilkinson, D. (CZ)
Palková, Z. (CZ)Number of authors 8 Article number 5597 Source Title International Journal of Molecular Sciences. - : MDPI
Roč. 22, č. 11 (2021)Number of pages 16 s. Language eng - English Country CH - Switzerland Keywords mitochondrial retrograde signaling ; yeast colonies ; colony development and differentiation ; Saccharomyces cerevisiae ; proteomic analysis Subject RIV EE - Microbiology, Virology OECD category Microbiology R&D Projects GA19-09381S GA ČR - Czech Science Foundation (CSF) Method of publishing Open access Institutional support MBU-M - RVO:61388971 UT WOS 000660155800001 EID SCOPUS 85106440628 DOI 10.3390/ijms22115597 Annotation During development of yeast colonies, various cell subpopulations form, which differ in their properties and specifically localize within the structure. Three branches of mitochondrial retrograde (RTG) signaling play a role in colony development and differentiation, each of them activating the production of specific markers in different cell types. Here, aiming to identify proteins and processes controlled by the RTG pathway, we analyzed proteomes of individual cell subpopulations from colonies of strains, mutated in genes of the RTG pathway. Resulting data, along with microscopic analyses revealed that the RTG pathway predominantly regulates processes in U cells, long-lived cells with unique properties, which are localized in upper colony regions. Rtg proteins therein activate processes leading to amino acid biosynthesis, including transport of metabolic intermediates between compartments, but also repress expression of mitochondrial ribosome components, thus possibly contributing to reduced mitochondrial translation in U cells. The results reveal the RTG pathway's role in activating metabolic processes, important in U cell adaptation to altered nutritional conditions. They also point to the important role of Rtg regulators in repressing mitochondrial activity in U cells. Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2022 Electronic address https://www.mdpi.com/1422-0067/22/11/5597/htm
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