Individual DNA Methylation Pattern Shifts in Nanoparticles-Exposed Workers Analyzed in Four Consecutive Years

Rössnerová, Andrea; Hoňková, Kateřina; Chvojková, Irena; Pelcová, D.; Ždímal, Vladimír; Hubáček, J. A.; Lischková, L.; Vlčková, Š.; Ondráček, Jakub; Dvořáčková, Š.; Topinka, Jan; Rössner ml., Pavel

doi:https://dx.doi.org/10.3390/ijms22157834

Number of the records: 1

Individual DNA Methylation Pattern Shifts in Nanoparticles-Exposed Workers Analyzed in Four Consecutive Years

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SYSNO ASEP	0545486
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Individual DNA Methylation Pattern Shifts in Nanoparticles-Exposed Workers Analyzed in Four Consecutive Years
Author(s)	Rössnerová, Andrea (UEM-P)_RID Hoňková, Kateřina (UEM-P) Chvojková, Irena (UEM-P) Pelcová, D. (CZ) Ždímal, Vladimír (UCHP-M)_{RID, ORCID, SAI} Hubáček, J. A. (CZ) Lischková, L. (CZ) Vlčková, Š. (CZ) Ondráček, Jakub (UCHP-M)_{RID, ORCID, SAI} Dvořáčková, Š. (CZ) Topinka, Jan (UEM-P)_{RID, ORCID} Rössner ml., Pavel (UEM-P)_{RID, ORCID}
Article number	7834
Source Title	International Journal of Molecular Sciences. - : MDPI Roč. 22, č. 15 (2021)
Number of pages	19 s.
Language	eng - English
Country	CH - Switzerland
Keywords	DNA methylation ; epigenetics ; human ; time changes ; microarrays ; nanoparticles ; occupational exposure
Subject RIV	DN - Health Impact of the Environment Quality
OECD category	Occupational health
Subject RIV - cooperation	Institute of Chemical Process Fundamentals - Health Impact of the Environment Quality
R&D Projects	GA18-02079S GA ČR - Czech Science Foundation (CSF)
Method of publishing	Open access
Institutional support	UEM-P - RVO:68378041 ; UCHP-M - RVO:67985858
UT WOS	000681888200001
EID SCOPUS	85110763922
DOI	10.3390/ijms22157834
Annotation	A DNA methylation pattern represents an original plan of the function settings of individual cells and tissues. The basic strategies of its development and changes during the human lifetime are known, but the details related to its modification over the years on an individual basis have not yet been studied. Moreover, current evidence shows that environmental exposure could generate changes in DNA methylation settings and, subsequently, the function of genes. In this study, we analyzed the effect of chronic exposure to nanoparticles (NP) in occupationally exposed workers repeatedly sampled in four consecutive years (2016-2019). A detailed methylation pattern analysis of 14 persons (10 exposed and 4 controls) was performed on an individual basis. A microarray-based approach using chips, allowing the assessment of more than 850 K CpG loci, was used. Individual DNA methylation patterns were compared by principal component analysis (PCA). The results show the shift in DNA methylation patterns in individual years in all the exposed and control subjects. The overall range of differences varied between the years in individual persons. The differences between the first and last year of examination (a three-year time period) seem to be consistently greater in the NP-exposed subjects in comparison with the controls. The selected 14 most differently methylated cg loci were relatively stable in the chronically exposed subjects. In summary, the specific type of long-term exposure can contribute to the fixing of relevant epigenetic changes related to a specific environment as, e.g., NP inhalation.
Workplace	Institute of Experimental Medicine
Contact	Lenka Koželská, lenka.kozelska@iem.cas.cz, Tel.: 241 062 218, 296 442 218
Year of Publishing	2022
Electronic address	https://www.mdpi.com/1422-0067/22/15/7834

Number of the records: 1