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Structure elucidation of the novel carotenoid gemmatoxanthin from the photosynthetic complex of Gemmatimonas phototrophica AP64
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SYSNO ASEP 0544992 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Structure elucidation of the novel carotenoid gemmatoxanthin from the photosynthetic complex of Gemmatimonas phototrophica AP64 Author(s) Kuzma, Marek (MBU-M) ORCID, RID
Hájek, Jan (MBU-M) ORCID
Hrouzek, Pavel (MBU-M) ORCID
Gardiner, Alastair T. (MBU-M) ORCID
Lukeš, Martin (MBU-M) ORCID
Moos, Martin (BC-A) RID, ORCID
Šimek, Petr (BC-A) RID, ORCID
Koblížek, Michal (MBU-M) RID, ORCID
Nupur, Nupur (MBU-M) ORCIDArticle number 15964 Source Title Scientific Reports. - : Nature Publishing Group - ISSN 2045-2322
Roč. 11, č. 1 (2021)Number of pages 12 s. Language eng - English Country DE - Germany Keywords energy-transfer ; biosynthetic-pathway ; ft-ir ; raman ; bacteriochlorophyll ; evolution ; strain ; synechoxanthin ; thiorhodaceae ; spectroscopy Subject RIV EE - Microbiology, Virology OECD category Microbiology Subject RIV - cooperation Biology Centre (since 2006) - Biochemistry R&D Projects GX19-28778X GA ČR - Czech Science Foundation (CSF) Method of publishing Open access Institutional support MBU-M - RVO:61388971 ; BC-A - RVO:60077344 UT WOS 000684439300023 EID SCOPUS 85112619992 DOI 10.1038/s41598-021-95254-6 Annotation Gemmatimonas phototrophica AP64 is the first phototrophic representative of the bacterial phylum Gemmatimonadetes. The cells contain photosynthetic complexes with bacteriochlorophyll a as the main light-harvesting pigment and an unknown carotenoid with a single broad absorption band at 490 nm in methanol. The carotenoid was extracted from isolated photosynthetic complexes, and purified by liquid chromatography. A combination of nuclear magnetic resonance (H-1 NMR, COSY, H-1-C-13 HSQC, H-1-C-13 HMBC, J-resolved, and ROESY), high-resolution mass spectroscopy, Fourier-transformed infra-red, and Raman spectroscopy was used to determine its chemical structure. The novel linear carotenoid, that we have named gemmatoxanthin, contains 11 conjugated double bonds and is further substituted by methoxy, carboxyl and aldehyde groups. Its IUPAC-IUBMB semi-systematic name is 1 'Methoxy-19 'oxo-3 ' ,4 'didehydro-7,8,1 ' ,2 'tetrahydro- Psi, Psi carotene-16-oic acid. To our best knowledge, the presence of the carboxyl, methoxy and aldehyde groups on a linear C40 carotenoid backbone is reported here for the first time. Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2022 Electronic address https://www.nature.com/articles/s41598-021-95254-6
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