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Biological autoluminescence as a noninvasive monitoring tool for chemical and physical modulation of oxidation in yeast cell culture
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SYSNO ASEP 0543278 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Biological autoluminescence as a noninvasive monitoring tool for chemical and physical modulation of oxidation in yeast cell culture Author(s) Bereta, M. (SK)
Teplan, M. (SK)
Chafai, Djamel Eddine (URE-Y)
Radil, R. (SK)
Cifra, Michal (URE-Y) RID, ORCID, SAINumber of authors 4 Article number 328 Source Title Scientific Reports. - : Nature Publishing Group - ISSN 2045-2322
Roč. 11, č. 1 (2021)Number of pages 11 s. Publication form Print - P Language eng - English Country GB - United Kingdom Keywords cell culture technique ; oxidation reduction reaction ; Saccharomyces cerevisiae Subject RIV BO - Biophysics OECD category Biophysics R&D Projects GA18-23597S GA ČR - Czech Science Foundation (CSF) Method of publishing Open access Institutional support URE-Y - RVO:67985882 UT WOS 000627829300032 EID SCOPUS 85099207559 DOI 10.1038/s41598-020-79668-2 Annotation Normal or excessive oxidative metabolism in organisms is essential in physiological and pathophysiological processes, respectively. Therefore, monitoring of biological oxidative processes induced by the chemical or physical stimuli is nowadays of extreme importance due to the environment overloaded with various physicochemical factors. Current techniques typically require the addition of chemical labels or light illumination, which perturb the samples to be analyzed. Moreover, the current techniques are very demanding in terms of sample preparation and equipment. To alleviate these limitations, we propose a label-free monitoring tool of oxidation based on biological autoluminescence (BAL). We demonstrate this tool on Saccharomyces cerevisiae cell culture. We showed that BAL can be used to monitor chemical perturbation of yeast due to Fenton reagents initiated oxidation-the BAL intensity changes with hydrogen peroxide concentration in a dose-dependent manner. Furthermore, we also showed that BAL reflects the effects of low-frequency magnetic field on the yeast cell culture, where we observed a disturbance of the BAL kinetics in the exposed vs. control case. Our results contribute to the development of novel techniques for label-free, real-time, noninvasive monitoring of oxidative processes and approaches for their modulation Workplace Institute of Radio Engineering and Electronics Contact Petr Vacek, vacek@ufe.cz, Tel.: 266 773 413, 266 773 438, 266 773 488 Year of Publishing 2022 Electronic address https://doi.org/10.1038/s41598-020-79668-2
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