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Matriptase-2 and Hemojuvelin in Hepcidin Regulation: In Vivo Immunoblot Studies in Mask Mice
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SYSNO ASEP 0542251 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Matriptase-2 and Hemojuvelin in Hepcidin Regulation: In Vivo Immunoblot Studies in Mask Mice Author(s) Krijt, J. (CZ)
Frýdlová, J. (CZ)
Gurieva, I. (CZ)
Přikryl, P. (CZ)
Báječný, M. (CZ)
Steinbicker, A. U. (DE)
Vokurka, M. (CZ)
Truksa, Jaroslav (BTO-N) RID, ORCIDNumber of authors 8 Article number 2650 Source Title International Journal of Molecular Sciences. - : MDPI
Roč. 22, č. 5 (2021)Number of pages 16 s. Language eng - English Country CH - Switzerland Keywords hepcidin ; transferrin receptor ; neogenin ; Hjv Subject RIV EB - Genetics ; Molecular Biology OECD category Biochemistry and molecular biology R&D Projects GA18-13103S GA ČR - Czech Science Foundation (CSF) LQ1604 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) ED1.1.00/02.0109 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support BTO-N - RVO:86652036 UT WOS 000628250600001 EID SCOPUS 85101964197 DOI 10.3390/ijms22052650 Annotation Matriptase-2, a serine protease expressed in hepatocytes, is a negative regulator of hepcidin expression. The purpose of the study was to investigate the interaction of matriptase-2 with hemojuvelin protein in vivo. Mice lacking the matriptase-2 proteolytic activity (mask mice) display decreased content of hemojuvelin protein. Vice versa, the absence of hemojuvelin results in decreased liver content of matriptase-2, indicating that the two proteins interact. To further characterize the role of matriptase-2, we investigated iron metabolism in mask mice fed experimental diets. Administration of iron-enriched diet increased liver iron stores as well as hepcidin expression. Treatment of iron-overloaded mask mice with erythropoietin increased hemoglobin and hematocrit, indicating that the response to erythropoietin is intact in mask mice. Feeding of an iron-deficient diet to mask mice significantly increased spleen weight as well as the splenic content of erythroferrone and transferrin receptor proteins, indicating stress erythropoiesis. Liver hepcidin expression was decreased, expression of Id1 was not changed. Overall, the results suggest a complex interaction between matriptase-2 and hemojuvelin, and demonstrate that hepcidin can to some extent be regulated even in the absence of matriptase-2 proteolytic activity. Workplace Institute of Biotechnology Contact Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700 Year of Publishing 2022 Electronic address https://www.mdpi.com/1422-0067/22/5/2650
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