Number of the records: 1
Multiprotein Complexes
- 1.
SYSNO ASEP 0537940 Document Type M - Monograph Chapter R&D Document Type Monograph Chapter Title Studying Protein-DNA Interactions by Hydrogen/Deuterium Exchange Mass Spectrometry Author(s) Filandrová, Růžena (MBU-M) ORCID
Kavan, Daniel (MBU-M) RID, ORCID
Kádek, Alan (MBU-M) RID, ORCID
Novák, Petr (MBU-M) RID, ORCID
Man, Petr (MBU-M) RID, ORCIDSource Title Multiprotein Complexes, Methods and Protocols, 2247. - New York : Springer, 2021 / Poterszman Arnaud - ISBN 978-1-0716-1125-8 Pages s. 193-219 Number of pages 27 s. Number of pages 340 Publication form Print - P Language eng - English Country US - United States Keywords DNA ; Hydrogen/deuterium exchange ; Protein–DNA binding ; Structural mass spectrometry ; Transcription factor Subject RIV CE - Biochemistry OECD category Biochemistry and molecular biology R&D Projects GA16-24309S GA ČR - Czech Science Foundation (CSF) GA16-20860S GA ČR - Czech Science Foundation (CSF) LQ1604 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) ED1.1.00/02.0109 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LQ1604 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support MBU-M - RVO:61388971 UT WOS 000685659700012 EID SCOPUS 85097844417 DOI 10.1007/978-1-0716-1126-5_11 Annotation Protein hydrogen/deuterium exchange (HDX) coupled to mass spectrometry (MS) can be used to study interactions of proteins with various ligands, to describe the effects of mutations, or to reveal structural responses of proteins to different experimental conditions. It is often described as a method with virtually no limitations in terms of protein size or sample composition. While this is generally true, there are, however, ligands or buffer components that can significantly complicate the analysis. One such compound, that can make HDX-MS troublesome, is DNA. In this chapter, we will focus on the analysis of protein–DNA interactions, describe the detailed protocol, and point out ways to overcome the complications arising from the presence of DNA. Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2021 Electronic address https://link.springer.com/protocol/10.1007/978-1-0716-1126-5_11
Number of the records: 1