Number of the records: 1
Follicle-stimulating hormone administration affects amino acid metabolism in mammalian oocytes
- 1.
SYSNO ASEP 0537765 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Follicle-stimulating hormone administration affects amino acid metabolism in mammalian oocytes Author(s) Tětková, Anna (UZFG-Y) ORCID
Šušor, Andrej (UZFG-Y) RID, ORCID
Kubelka, Michal (UZFG-Y) RID, ORCID
Němcová, Lucie (UZFG-Y) RID
Jansová, Denisa (UZFG-Y) ORCID
Dvořan, Michal (UZFG-Y) ORCID
del Llano, Edgar (UZFG-Y) ORCID
Holubcová, Z. (CZ)
Kalous, Jaroslav (UZFG-Y) RID, ORCIDSource Title Biology of Reproduction. - : Oxford University Press - ISSN 0006-3363
Roč. 101, č. 4 (2019), s. 719-732Number of pages 14 s. Publication form Print - P Language eng - English Country US - United States Keywords oocyte ; oocyte maturation ; FSH/FSH receptor Subject RIV EB - Genetics ; Molecular Biology OECD category Biochemistry and molecular biology R&D Projects EF15_003/0000460 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GA18-19395S GA ČR - Czech Science Foundation (CSF) GA19-13491S GA ČR - Czech Science Foundation (CSF) Method of publishing Limited access Institutional support UZFG-Y - RVO:67985904 UT WOS 000509503400008 EID SCOPUS 85075813083 DOI 10.1093/biolre/ioz117 Annotation Culture media used in assisted reproduction are commonly supplemented with gonadotropin hormones to support the nuclear and cytoplasmic maturation of in vitro matured oocytes. However, the effect of gonadotropins on protein synthesis in oocytes is yet to be fully understood. As published data have previously documented a positive in vitro effect of follicle-stimulating hormone (FSH) on cytoplasmic maturation, we exposed mouse denuded oocytes to FSH in order to evaluate the changes in global protein synthesis. We found that dose-dependent administration of FSH resulted in a decrease of methionine incorporation into de novo synthesized proteins in denuded mouse oocytes and oocytes cultured in cumulus-oocyte complexes. Similarly, FSH influenced methionine incorporation in additional mammalian species including human. Furthermore, we showed the expression of FSH-receptor protein in oocytes. We found that major translational regulators were not affected by FSH treatment, however, the amino acid uptake became impaired. We propose that the effect of FSH treatment on amino acid uptake is influenced by FSH receptor with the effect on oocyte metabolism and physiology. Workplace Institute of Animal Physiology and Genetics Contact Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Year of Publishing 2021 Electronic address https://asep.lib.cas.cz/arl-cav/cs/csg/?repo=crepo1&key=86555598611
Number of the records: 1