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DNA damage induced by occupational exposure to copper oxide nanoparticles
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SYSNO ASEP 0532785 Document Type C - Proceedings Paper (int. conf.) R&D Document Type Conference Paper Title DNA damage induced by occupational exposure to copper oxide nanoparticles Author(s) Rössner st., Pavel (UEM-P)
Pelcová, D. (CZ)
Elzeinová, Fatima (UEM-P)
Mikuška, Pavel (UIACH-O) RID, ORCID
Večeřa, Zbyněk (UIACH-O) RID
Coufalík, Pavel (UIACH-O) ORCID, RID
Vlčková, Š. (CZ)
Fenclová, Z. (CZ)
Rössnerová, Andrea (UEM-P) RIDNumber of authors 9 Source Title NANOCON Conference Proceedings - International Conference on Nanomaterials, Volume 2020-October. - Ostrava : Tanger Ltd., 2020 - ISSN 2694-930X - ISBN 978-808729495-6 Pages s. 486-490 Number of pages 5 s. Publication form Online - E Action NANOCON 2019. International Conference on Nanomaterials - Research & Application /11./ Event date 16.10.2019 - 18.10.2019 VEvent location Brno Country CZ - Czech Republic Event type EUR Language eng - English Country CZ - Czech Republic Keywords copper oxide nanoparticles ; inhalation ; DNA damage Subject RIV CB - Analytical Chemistry, Separation OECD category Analytical chemistry Subject RIV - cooperation Institute of Experimental Medicine R&D Projects GA18-02079S GA ČR - Czech Science Foundation (CSF) LO1508 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UIACH-O - RVO:68081715 ; UEM-P - RVO:68378041 UT WOS 000664115400083 EID SCOPUS 85097192165 DOI 10.37904/nanocon.2019.8653 Annotation Copper oxide nanoparticles (CuO NPs) have a widespread use in industry, chemistry, in production of electronic devices and as an antimicrobial agent. Although copper is an important biogenic element, CuO NPs are toxic with the ability to induce oxidative stress, apoptosis, cell cycle arrest or DNA damage. For humans, the inhalation route is the most common way of exposure to CuO NPs. In the body, CuO NPs may be either deposited in the lungs, or transported to other organs. Their presence usually causes oxidative stress or inflammatory responses, consequently leading to DNA damage. In this study, we investigated the effect of CuO NPs inhalation on DNA damage in a group of researches conducting animal exposure experiments. The subjects were exposed to various metal oxide nanoparticles, including CuO NPs, by inhalation for an average of 4.9 ± 0.4 years. The average mass concentration of Cu in the air during the experiment was 7.3 ± 3.2 ng/m3. Subjects not exposed to nanoparticles served as a control group. We applied micronucleus assay using Human Pan Centromeric probes to detect DNA damage and to distinguish between the frequency of centromere positive (CEN+) and centromere negative (CEN−) micronuclei (MN) in the binucleated cells. We
did not find differences between both groups for either mean MN frequency (10.38 ± 2.50 vs. 11.88 ± 3.01 MN/1000 binucleated cells), or CEN+/CEN- ratio (58%/42% vs. 55%/45%), for the exposed and controls, respectively. In conclusion, inhalation of CuO NPs at this low-level exposure had no effect on chromosomal losses and/or breaks.Workplace Institute of Analytical Chemistry Contact Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234 Year of Publishing 2021 Electronic address https://www.confer.cz/nanocon/2019
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