Bioengineering a pre-vascularized pouch for subsequent islet transplantation using VEGF-loaded polylactide capsules

Kasoju, Naresh; Pátiková, A.; Wawrzyńska, Edyta; Vojtíšková, A.; Sedlačík, Tomáš; Kumorek, Marta M.; Pop-Georgievski, Ognen; Sticová, E.; Kříž, J.; Kubies, Dana

doi:https://dx.doi.org/10.1039/C9BM01280J

Number of the records: 1

Bioengineering a pre-vascularized pouch for subsequent islet transplantation using VEGF-loaded polylactide capsules

1.

SYSNO ASEP	0520757
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Bioengineering a pre-vascularized pouch for subsequent islet transplantation using VEGF-loaded polylactide capsules
Author(s)	Kasoju, Naresh (UMCH-V)_{RID, ORCID} Pátiková, A. (CZ) Wawrzyńska, Edyta (UMCH-V)_{RID, ORCID} Vojtíšková, A. (CZ) Sedlačík, Tomáš (UMCH-V)_RID Kumorek, Marta M. (UMCH-V)_RID Pop-Georgievski, Ognen (UMCH-V)_{RID, ORCID} Sticová, E. (CZ) Kříž, J. (CZ) Kubies, Dana (UMCH-V)_{RID, ORCID}
Source Title	Biomaterials Science . - : Royal Society of Chemistry - ISSN 2047-4830 Roč. 8, č. 2 (2020), s. 631-647
Number of pages	17 s.
Language	eng - English
Country	GB - United Kingdom
Keywords	surface functionalization ; PLA ; scaffold
Subject RIV	CD - Macromolecular Chemistry
OECD category	Polymer science
R&D Projects	NV16-28254A GA MZd - Ministry of Health (MZ)
	LQ1604 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	ED1.1.00/02.0109 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Method of publishing	Open access
Institutional support	UMCH-V - RVO:61389013
UT WOS	000509549500007
EID SCOPUS	85078378760
DOI	10.1039/C9BM01280J
Annotation	he effectiveness of cell transplantation can be improved by optimization of the transplantation site. For some types of cells that form highly oxygen-demanding tissue, e.g., pancreatic islets, a successful engraftment depends on immediate and sufficient blood supply. This critical point can be avoided when cells are transplanted into a bioengineered pre-vascularized cavity which can be formed using a polymer scaffold. In our study, we tested surface-modified poly(lactide-co-caprolactone) (PLCL) capsular scaffolds containing the pro-angiogenic factor VEGF. After each modification step (i.e., amination and heparinization), the surface properties and morphology of scaffolds were characterized by ATR-FTIR and XPS spectroscopy, and by SEM and AFM. All modifications preserved the gross capsule morphology and maintained the open pore structure. Optimized aminolysis conditions decreased the Mw of PLCL only up to 10% while generating a sufficient number of NH2 groups required for the covalent immobilization of heparin. The heparin layer served as a VEGF reservoir with an in vitro VEGF release for at least four weeks. In vivo studies revealed that to obtain highly vascularized PLCL capsules (a) the optimal VEGF dose for the capsule was 50 μg and (b) the implantation time was four weeks when implanted into the greater omentum of Lewis rats. Dense fibrous tissue accompanied by vessels completely infiltrated the scaffold and created sparse granulation tissue within the internal cavity of the capsule. The prepared pre-vascularized pouch enabled the islet graft survival and functioning for at least 50 days after islet transplantation. The proposed construct can be used to create a reliable pre-vascularized pouch for cell transplantation.
Workplace	Institute of Macromolecular Chemistry
Contact	Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358
Year of Publishing	2021
Electronic address	https://pubs.rsc.org/en/content/articlelanding/2020/BM/C9BM01280J#!divAbstract

Number of the records: 1