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Transcriptome, proteome and draft genome of Euglena gracilis
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SYSNO ASEP 0519415 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Transcriptome, proteome and draft genome of Euglena gracilis Author(s) Ebenezer, N.D. (GB)
Zoltner, M. (GB)
Burrell, A. (GB)
Nenarokova, Anna (BC-A) RID, ORCID
Vanclová-Novák, A.M.G. (CZ)
Prasad, B. (DE)
Soukal, P. (CZ)
Santana-Molina, C. (ES)
O'Neill, E. (GB)
Nankissoor, N.N. (CA)
Vadakedath, N. (DE)
Daiker, V. (DE)
Obado, S. (US)
Silva-Pereira, S. (GB)
Jackson, A.P. (GB)
Devos, D.P. (ES)
Lukeš, Julius (BC-A) RID, ORCID
Lebert, M. (DE)
Vaughan, S. (GB)
Hampl, V. (CZ)
Carrington, M. (GB)
Ginger, M. L. (GB)
Dacks, J.B. (CA)
Kelly, S. (GB)
Field, Mark Christian (BC-A) ORCIDNumber of authors 25 Article number 11 Source Title BMC BIOLOGY. - : BioMed Central
Roč. 17, FEB 7 2019 (2019)Number of pages 23 s. Publication form Online - E Language eng - English Country GB - United Kingdom Keywords trypanosoma-brucei ; gene-expression ; signal-transduction ; likely common ; tubulin genes ; beta-tubulin ; sequence ; evolution ; biosynthesis ; annotation ; Euglena gracilis ; Transcriptome ; Cellular evolution ; Plastid ; Horizontal gene transfer ; Gene architecture ; Splicing ; Secondary endosymbiosis ; Excavata Subject RIV EH - Ecology, Behaviour OECD category Ecology R&D Projects LL1601 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) EF16_019/0000759 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support BC-A - RVO:60077344 UT WOS 000458128000001 EID SCOPUS 85061200138 DOI 10.1186/s12915-019-0626-8 Annotation BackgroundPhotosynthetic euglenids are major contributors to fresh water ecosystems. Euglena gracilis in particular has noted metabolic flexibility, reflected by an ability to thrive in a range of harsh environments. E. gracilis has been a popular model organism and of considerable biotechnological interest, but the absence of a gene catalogue has hampered both basic research and translational efforts.ResultsWe report a detailed transcriptome and partial genome for E. gracilis Z1. The nuclear genome is estimated to be around 500Mb in size, and the transcriptome encodes over 36,000 proteins and the genome possesses less than 1% coding sequence. Annotation of coding sequences indicates a highly sophisticated endomembrane system, RNA processing mechanisms and nuclear genome contributions from several photosynthetic lineages. Multiple gene families, including likely signal transduction components, have been massively expanded. Alterations in protein abundance are controlled post-transcriptionally between light and dark conditions, surprisingly similar to trypanosomatids.ConclusionsOur data provide evidence that a range of photosynthetic eukaryotes contributed to the Euglena nuclear genome, evidence in support of the shopping bag' hypothesis for plastid acquisition. We also suggest that euglenids possess unique regulatory mechanisms for achieving extreme adaptability, through mechanisms of paralog expansion and gene acquisition. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2020 Electronic address https://bmcbiol.biomedcentral.com/track/pdf/10.1186/s12915-019-0626-8
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