”Salicylic Acid Mutant Collection“ as a Tool to Explore the Role of Salicylic Acid in Regulation of Plant Growth under a Changing Environment

0518894 - ÚEB 2020 RIV CH eng J - Journal Article
Pluhařová, Kamila - Leontovyčová, Hana - Stoudková, Věra - Pospíchalová, Romana - Maršík, P. - Klouček, P. - Starodubtseva, Anastasiia - Iakovenko, Oksana - Krčková, Zuzana - Valentová, O. - Burketová, Lenka - Janda, Martin - Kalachova, Tetiana
”Salicylic Acid Mutant Collection“ as a Tool to Explore the Role of Salicylic Acid in Regulation of Plant Growth under a Changing Environment.
International Journal of Molecular Sciences. Roč. 20, č. 24 (2019), č. článku 6365. E-ISSN 1422-0067
R&D Projects: GA ČR GA17-05151S; GA MŠMT(CZ) EF16_019/0000738
Grant - others:PPPLZ(CZ) TK919220
Institutional support: RVO:61389030
Keywords : Arabidopsis mutants * gene transcription * growth * light * Salicylic acid
OECD category: Biochemical research methods
Impact factor: 4.556, year: 2019
Method of publishing: Open access
http://dx.doi.org/10.3390/ijms20246365

The phytohormone salicylic acid (SA) has a crucial role in plant physiology. Its role is best described in the context of plant response to pathogen attack. During infection, SA is rapidly accumulated throughout the green tissues and is important for both local and systemic defences. However, some genetic/metabolic variations can also result in SA overaccumulation in plants, even in basal conditions. To date, more than forty Arabidopsis thaliana mutants have been described as having enhanced endogenous SA levels or constitutively activated SA signalling pathways. In this study, we established a collection of mutants containing different SA levels due to diverse genetic modifications and distinct gene functions. We chose prototypic SA-overaccumulators (SA-OAs), such as bon1-1, but also non-typical ones such as exo70b1-1, the selection of OA is accompanied by their crosses with SA-deficient lines. Here, we extensively studied the plant development and SA level/signalling under various growth conditions in soil and in vitro, and showed a strong negative correlation between rosette size, SA content and PR1/ICS1 transcript signature. SA-OAs (namely cpr5, acd6, bon1-1, fah1/fah2 and pi4kβ1β2) had bigger rosettes under high light conditions, whereas WT plants did not. Our data provide new insights clarifying a link between SA and plant behaviour under environmental stresses. The presented SA mutant collection is thus a suitable tool to shed light on the mechanisms underlying trade-offs between growth and defence in plants.
Permanent Link: http://hdl.handle.net/11104/0303905