Evaluating amplicon high-throughput sequencing data of microalgae living in melting snow: improvements and limitations

0511794 - BÚ 2020 RIV CZ eng J - Journal Article
Lutz, S. - Procházková, L. - Benning, L. G. - Nedbalová, Linda - Remias, D.
Evaluating amplicon high-throughput sequencing data of microalgae living in melting snow: improvements and limitations.
Fottea. Roč. 19, č. 2 (2019), s. 115-131. ISSN 1802-5439. E-ISSN 1805-4927
Institutional support: RVO:67985939
Keywords : 18S rDNA * ITS2 rDNA * snow algae
OECD category: Plant sciences, botany
Impact factor: 2.465, year: 2019
Method of publishing: Open access

Our evaluation reveals that HTS outputs need to be thoroughly checked when the organisms are poorly represented in databases, which is also the case of snow algae. We recommend an optimized workflow including consistent sampling, a two–molecular marker approach, light microscopy–based guidance, generation of appropriate reference sequences and a final manual verification of taxonomic assignments as a best approach for accurate diversity analyses.
Permanent Link: http://hdl.handle.net/11104/0302041