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Whey Protein Complexes with Green Tea Polyphenols: Antimicrobial, Osteoblast-Stimulatory, and Antioxidant Activities
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SYSNO ASEP 0505670 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Whey Protein Complexes with Green Tea Polyphenols: Antimicrobial, Osteoblast-Stimulatory, and Antioxidant Activities Author(s) Carson, M. (GB)
Keppler, J. K. (DE)
Brackman, G. (BE)
Dawood, D. (DE)
Vandrovcová, Marta (FGU-C) RID, ORCID
El-Sayed, K. F. (DE)
Coenye, T. (BE)
Schwarz, K. (DE)
Clarke, S. A. (GB)
Skirtach, A. G. (BE)
Douglas, T.E.L. (BE)Source Title Cells Tissues Organs. - : Karger - ISSN 1422-6405
Roč. 206, 1-2 (2018), s. 106-117Number of pages 12 s. Language eng - English Country CH - Switzerland Keywords whey protein isolate ; polyphenol ; osteoblast ; antibacterial properties ; antioxidant Subject RIV EI - Biotechnology ; Bionics OECD category Technologies involving identifying the functioning of DNA, proteins and enzymes and how they influence the onset of disease and maintenance of well-being (gene-based diagnostics and therapeutic interventions (pharmacogenomics, gene-based therapeutics) Method of publishing Limited access Institutional support FGU-C - RVO:67985823 UT WOS 000467644100014 EID SCOPUS 85060651847 DOI 10.1159/000494732 Annotation Polyphenols are known for their antimicrobial activity, whilst both polyphenols and the globular protein beta-lactoglobulin (bLG) are suggested to have antioxidant properties and promote cell proliferation. These are potentially useful properties for a tissue-engineered construct, though it is unknown if they are retained when both compounds are used in combination. In this study, a range of different microbes and an osteoblast-like cell line (human fetal osteoblast, hFOB) were used to assess the combined effect of: (1) green tea extract (GTE), rich in the polyphenol epigallocatechin gallate (EGCG), and (2) whey protein isolate (WPI), rich in bLG. It was shown that approximately 20-48% of the EGCG in GTE reacted with WPI. GTE inhibited the growth of Gram-positive bacteria, an effect which was potentiated by the addition of WPI. GTE alone also significantly inhibited the growth of hFOB cells after 1, 4, and 7 days of culture. Alternatively, WPI significantly promoted hFOB cell growth in the absence of GTE and attenuated the effect of GTE at low concentrations (64 mu g/mL) after 4 and 7 days. Low concentrations of WPI (50 mu g/mL) also promoted the expression of the early osteogenic marker alkaline phosphatase (ALP) by hFOB cells, whereas GTE inhibited ALP activity. Therefore, the antioxidant effects of GTE can be boosted by WPI, but GTE is not suitable to be used as part of a tissue-engineered construct due to its cytotoxic effects which negate any positive effect WPI has on cell proliferation. Workplace Institute of Physiology Contact Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Year of Publishing 2020 Electronic address https://www.karger.com/Article/Abstract/494732
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