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Capillary electrophoresis with preparative isoelectric focusing preconcentration for sensitive determination of amphotericin B in human blood serum
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SYSNO ASEP 0501010 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Capillary electrophoresis with preparative isoelectric focusing preconcentration for sensitive determination of amphotericin B in human blood serum Author(s) Šalplachta, Jiří (UIACH-O) RID, ORCID
Horká, Marie (UIACH-O) RID, ORCID
Šlais, Karel (UIACH-O) RID, ORCIDNumber of authors 3 Source Title Analytica Chimica Acta. - : Elsevier - ISSN 0003-2670
Roč. 1053, APR (2019), s. 162-168Number of pages 7 s. Publication form Print - P Language eng - English Country NL - Netherlands Keywords capillary zone electrophoresis ; Amphotericin B ; blood ; sample preparation Subject RIV CB - Analytical Chemistry, Separation OECD category Analytical chemistry R&D Projects GA16-03749S GA ČR - Czech Science Foundation (CSF) NV16-29916A GA MZd - Ministry of Health (MZ) VI20172020069 GA MV - Ministry of Interior (MV) Method of publishing Limited access Institutional support UIACH-O - RVO:68081715 UT WOS 000457201600018 EID SCOPUS 85058795138 DOI 10.1016/j.aca.2018.12.010 Annotation Amphotericin B (AmB) is still, despite its severe nephrotoxicity, the first-line agent in the management of serious systemic fungal infections. A sensitive and reliable method is therefore required to control AmB concentration in body fluids of a patient. This study demonstrates the potential of the off-line combination of preparative isoelectric focusing (IEF) with capillary isoelectric focusing (CIEF) or capillary zone electrophoresis (CZE) in the determination of AmB in human blood serum. The required value of the isoelectric point of AmB was determined to be 6.1 using the CIEF technique. Preparative IEF served as a pre-separation and concentration technique. The pH gradient was traced by colored low molecular pI markers. The collected fraction with AmB was easily processed and then analyzed by CIEF and CZE. Tens of picograms of AmB in human blood serum sample can be determined by a combination of preparative IEF with CZE. The method was linear in the AmB concentration range of 0.3e600 ngmL 1. The recovery ranged from 93% to 98%. Workplace Institute of Analytical Chemistry Contact Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234 Year of Publishing 2020 Electronic address http://hdl.handle.net/11104/0293037
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