Metabolomic Profiling: Methods and Protocols, Methods in Molecular Biology

0493749 - BC 2019 RIV US eng M - Monography Chapter
Hušek, Petr - Švagera, Z. - Hanzlíková, D. - Karlínová, Iva - Řimnáčová, Lucie - Zahradníčková, Helena - Šimek, Petr
GC-MS metabolomic profiling of protic metabolites following heptafluorobutyl chloroformate mediated dispersive liquid microextraction sample preparation protocol.
Metabolomic Profiling: Methods and Protocols, Methods in Molecular Biology. Vol. 1738. New York: Humana Press, 2018 - (Theodoridis, G.; Gika, H.; Wilson, I.), s. 159-181. ISBN 978-1-4939-7642-3
R&D Projects: GA ČR(CZ) GA17-22276S
Institutional support: RVO:60077344
Keywords : metabolomic profiling * GC-MS * dispersive liquid-liquid microextraction
OECD category: Biochemical research methods

A simple analytical workflow is described for gas chromatographic-mass spectrometry (GC-MS)-based metabolomic profiling of protic metabolites, particularly amino-carboxylic species in biological matrices. The sample preparation is carried out directly in aqueous samples and uses simultaneous in situ heptafluorobutyl chloroformate (HFBCF) derivatization and dispersive liquid-liquid microextraction (DLLME), followed by GC-MS analysis in single-ion monitoring (SIM) mode. The protocol involves ten simple pipetting steps and provides quantitative analysis of 132 metabolites by using two internal standards. A comment on each analytical step and explaining notes are provided with particular attention to the GC-MS analysis of 112 physiological metabolites in human urine.
Permanent Link: http://hdl.handle.net/11104/0292225