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Comparison of two low flow interfaces for measurement of mobilities and stability constants by affinity capillary electrophoresis-mass spectrometry
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SYSNO ASEP 0493561 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Comparison of two low flow interfaces for measurement of mobilities and stability constants by affinity capillary electrophoresis-mass spectrometry Author(s) Konášová, Renáta (UOCHB-X) ORCID
Koval, Dušan (UOCHB-X) RID, ORCID
Jaklová Dytrtová, Jana (UOCHB-X) RID, ORCID
Kašička, Václav (UOCHB-X) RID, ORCIDSource Title Journal of Chromatography A. - : Elsevier - ISSN 0021-9673
Roč. 1568, Sep 21 (2018), s. 197-204Number of pages 8 s. Language eng - English Country NL - Netherlands Keywords affinity capillary electrophoresis ; CE-MS interface ; crown ether complexes ; electrospray ionization ; mass spectrometry ; stability constant Subject RIV CB - Analytical Chemistry, Separation OECD category Analytical chemistry R&D Projects GA15-01948S GA ČR - Czech Science Foundation (CSF) GA17-10832S GA ČR - Czech Science Foundation (CSF) GA17-12648S GA ČR - Czech Science Foundation (CSF) Institutional support UOCHB-X - RVO:61388963 UT WOS 000443669600021 EID SCOPUS 85050144898 DOI 10.1016/j.chroma.2018.07.014 Annotation Affinity capillary electrophoresis (ACE) is typically used for the determination of stability constant, K-st, of weak to moderately strong complexes. Sensitive detection such as mass spectrometry (MS) is required for extension of ACE methodology for estimation of K-st of stronger complexes. Consequently, an efficient interface for hyphenation of CE with MS detection is necessary. For evaluation of interfaces for electrospray ionization mass spectrometric (ESI/MS) detection in ACE conditions, potassium-crown ether complexation was used as model system. The effective mobilities of the crown ether ligands and the K-st of their potassium complexes were measured/determined by ACE-ESI/MS using two lab-made interfaces: (i) a sheathless porous tip CE-ESI/MS interface and (ii) a nano-sheath liquid flow CE-ESI/MS interface, and, in turn, compared with those obtained by ACE with UV spectrophotometric detection. Apparent stability constant of potassium-crown ether complexes in 60/40 (v/v) methanol/water mixed solvent, pH* 5.5, was about 1300 L/mol for dibenzo-18-crown-6, 1600 L/mol for benzo-18-crown-6 and 5200 L/mol for 18-crown-6 ligands, respectively. It was observed that electrophoretic mobilities from CE-MS experiments differ from reference values determined by UV detection by similar to 7% depending on the CE-MS interface used. Good agreement of CE-MS and CE-UV data was achieved for nano-sheath liquid flow interface, in which the spray potential and the CE separation potential can be effectively decoupled. As for sheathless porous tip interface, a correction procedure involving a mobility marker has been proposed. It provides typically only ca. 1% difference of effective mobilities and K-st values obtained from CE-MS data as compared to those received by the reference ACE-UV method. Workplace Institute of Organic Chemistry and Biochemistry Contact asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Jana Procházková, Tel.: 220 183 418 Year of Publishing 2019
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