Three-dimensional holographic optical manipulation through a high-numerical-aperture soft-glass multimode fibre

0489568 - ÚPT 2019 RIV GB eng J - Journal Article
Leite, I.T. - Turtaev, S. - Jiang, X. - Šiler, Martin - Cuschieri, A. - Russell, P. - Čižmár, Tomáš
Three-dimensional holographic optical manipulation through a high-numerical-aperture soft-glass multimode fibre.
Nature Photonics. Roč. 12, č. 1 (2018), s. 33-39. ISSN 1749-4885. E-ISSN 1749-4893
R&D Projects: GA MŠMT EF15_003/0000476
Institutional support: RVO:68081731
Keywords : photonic crystal fiber * fluorescence microscopy * scattering media
OECD category: Optics (including laser optics and quantum optics)
Impact factor: 31.583, year: 2018

Holographic optical tweezers (HOT) hold great promise for many applications in biophotonics, allowing the creation and measurement of minuscule forces on biomolecules, molecular motors and cells. Geometries used in HOT currently rely on bulk optics, and their exploitation in vivo is compromised by the optically turbid nature of tissues. We present an alternative HOT approach in which multiple three-dimensional (3D) traps are introduced through a high-numerical-aperture multimode optical fibre, thus enabling an equally versatile means of manipulation through channels having cross-section comparable to the size of a single cell. Our work demonstrates real-time manipulation of 3D arrangements of micro-objects, as well as manipulation inside otherwise inaccessible cavities. We show that the traps can be formed over fibre lengths exceeding 100 mm and positioned with nanometric resolution. The results provide the basis for holographic manipulation and other high-numerical-aperture techniques, including advanced microscopy, through single-core-fibre endoscopes deep inside living tissues and other complex environments.
Permanent Link: http://hdl.handle.net/11104/0283968