Molecular mechanisms of thermal resistance of the insect trypanosomatid Crithidia thermophilal

0485299 - BC 2018 RIV US eng J - Journal Article
Ishemgulova, A. - Butenko, A. - Kortišová, L. - Boucinha, C. - Grybchuk-Ieremenko, A. - Morelli, K.A. - Tesařová, Martina - Kraeva, N. - Grybchuk, D. - Pánek, T. - Flegontov, P. - Lukeš, Julius - Votýpka, Jan - Pavan, M. G. - Opperdoes, F. R. - Spodareva, V. - d'Avila-Levy, C.M. - Kostygov, A.Y. - Yurchenko, V.
Molecular mechanisms of thermal resistance of the insect trypanosomatid Crithidia thermophilal.
PLoS ONE. Roč. 12, č. 3 (2017), č. článku e0174165. ISSN 1932-6203. E-ISSN 1932-6203
R&D Projects: GA ČR(CZ) GA14-23986S; GA ČR(CZ) GA16-18699S
Institutional support: RVO:60077344
Keywords : subunit ribosomal-rna * sp-n * monoxenous trypanosomatids * neotropical heteroptera * mitochondrial hsp70 * paraflagellar rod * genus wallaceina * protein import * costa-rica * parasites
OECD category: Biochemistry and molecular biology
Impact factor: 2.766, year: 2017

In the present work, we investigated molecular mechanisms governing thermal resistance of a monoxenous trypanosomatid Crithidia luciliae thermophila, which we reclassified as a separate species C. thermophila. We analyzed morphology, growth kinetics, and transcriptomic profiles of flagellates cultivated at low (23 degrees C) and elevated (34 degrees C) temperature. When maintained at high temperature, they grew significantly faster, became shorter, with genes involved in sugar metabolism and mitochondrial stress protection significantly upregulated. Comparison with another thermoresistant monoxenous trypanosomatid, Leptomonas seymouri, revealed dramatic differences in transcription profiles of the two species with only few genes showing the same expression pattern. This disparity illustrates differences in the biology of these two parasites and distinct mechanisms of their thermotolerance, a prerequisite for living in warm-blooded vertebrates.
Permanent Link: http://hdl.handle.net/11104/0280367