Differences in spatial communities of European perch (Perca fluviatilis Linnaeus, 1758) fry in a canyon-shaped reservoir are not attributable to genetics

0479467 - BC 2018 RIV DE eng J - Journal Article
Kalous, L. - Kuříková, P. - Kohout, J. - Rylková, K. - Petrtýl, M. - Čech, Martin
Differences in spatial communities of European perch (Perca fluviatilis Linnaeus, 1758) fry in a canyon-shaped reservoir are not attributable to genetics.
Journal of Applied Ichthyology. Roč. 33, č. 2 (2017), s. 306-313. ISSN 0175-8659. E-ISSN 1439-0426.
[European Congress of Ichthyology /15./. Porto, 07.09.2015-11.09.2015]
R&D Projects: GA ČR GA206/06/1371; GA ČR GP206/09/P266; GA MŠMT(CZ) 7F14316
Institutional support: RVO:60077344
Keywords : diel vertical migration * cross-species amplification * dna-sequence data * postglacial colonization * microsatellite loci
OECD category: Marine biology, freshwater biology, limnology
Impact factor: 0.774, year: 2017

The question was whether a canyon-shaped reservoir is inhabited by genetically distinct populations of European perch Perca fluviatilis exhibiting different spatial behaviour, or by a single population. The study was conducted in the spring of 2006 at Rimov Reservoir, Czech Republic. Epipelagic and bathypelagic perch fry (EPF and BPF) were captured by trawl net (mesh size 1x1.35mm) controlled by echo sounder, and littoral perch fry and adults (LPF and LPA) were caught by electrofishing and beach seine (mesh size 1x1.35mm). DNA was isolated in 266 individuals from the reservoir, and phylogenetic analysis based on mtDNA was performed (n=18) on BPF, EPF, LPF, and LPA from the reservoir and additional sequences from Central Europe. Six microsatellites were used for differentiating among communities (n=266). There was no evidence for genetic divergence in mtDNA sequences. Analyses of microsatellites revealed no statistically significant variations between spatial communities, and confirmed genetic homogeneity among communities. F-ST values and the number of genetic clusters point to the same conclusion, i.e., the reservoir is inhabited by a single population whose individuals occupy different microhabitats.
Permanent Link: http://hdl.handle.net/11104/0275467