Effect of N-Feruloylserotonin and Methotrexate on Severity of Experimental Arthritis and on Messenger RNA Expression of Key Proinflammatory Markers in Liver

0463455 - ÚOCHB 2017 RIV GB eng J - Journal Article
Pašková, L. - Kuncírová, V. - Poništ, S. - Mihálová, D. - Nosál, R. - Harmatha, Juraj - Hrádková, I. - Čavojský, T. - Bilka, F. - Šišková, K. - Paulíková, I. - Bezáková, L. - Bauerová, K.
Effect of N-Feruloylserotonin and Methotrexate on Severity of Experimental Arthritis and on Messenger RNA Expression of Key Proinflammatory Markers in Liver.
Journal of Immunology Research. Roč. 2016, June (2016), č. článku 7509653. ISSN 2314-8861. E-ISSN 2314-7156
Institutional support: RVO:61388963
Keywords : rheumatoid arthritis * chronic inflammatory disease * serotonin phenylpropanoids
Subject RIV: FR - Pharmacology ; Medidal Chemistry
Impact factor: 3.276, year: 2016
https://www.hindawi.com/journals/jir/2016/7509653/

Rheumatoid arthritis (RA) is a chronic inflammatory disease, leading to progressive destruction of joints and extra-articular tissues, including organs such as liver and spleen. The purpose of this study was to compare the effects of a potential immunomodulator, natural polyphenol N-feruloylserotonin (N-f-5HT), with methotrexate (MTX), the standard in RA therapy, in the chronic phase of adjuvant-induced arthritis (AA) in male Lewis rats. The experiment included healthy controls (CO), arthritic animals (AA), AA given N-f-5HT (AA-N-f-5HT), and AA given MTX (AA-MTX). N-f-5HT did not affect the body weight change and clinical parameters until the 14th experimental day. Its positive effect was rising during the 28-day experiment, indicating a delayed onset of N-f-5HT action. Administration of either N-f-5HT or MTX caused reduction of inflammation measured as the level of CRP in plasma and the activity of LOX in the liver. mRNA transcription of TNF-.. and iNOS in the liver was significantly attenuated in both MTX and N-f-5HT treated groups of arthritic rats. Interestingly, in contrast to MTX, N-f-5HT significantly lowered the level of IL-1.. in plasma and IL-1 beta mRNA expression in the liver and spleen of arthritic rats. This speaks for future investigations of N-f-5HT as an agent in the treatment of RA in combination therapy with MTX.
Permanent Link: http://hdl.handle.net/11104/0262649