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Modified Nucleic Acids
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SYSNO ASEP 0461380 Document Type M - Monograph Chapter R&D Document Type Monograph Chapter Title Polymerase Synthesis of Base-Modified DNA Author(s) Daďová, Jitka (UOCHB-X) RID
Cahová, Hana (UOCHB-X) RID, ORCID
Hocek, Michal (UOCHB-X) RID, ORCIDSource Title Modified Nucleic Acids. - Cham : Springer, 2016 / Nakatani K. ; Tor Y. - ISSN 0933-1891 - ISBN 978-3-319-27109-5 Pages s. 123-144 Number of pages 22 s. Number of pages 276 Publication form Print - P Language eng - English Country CH - Switzerland Keywords enzyme amplification reaction ; cross-coupling reactions ; labeled nucleoside triphosphonates Subject RIV CC - Organic Chemistry Institutional support UOCHB-X - RVO:61388963 UT WOS 000376296500007 DOI 10.1007/978-3-319-27111-8_6 Annotation Enzymatic synthesis of base-modified DNA by polymerase incorporation of modified nucleotides is discussed. Modified 20-deoxyribonucleoside triphosphates (dNTPs) are key substrates for polymerases and can be prepared either by triphosphorylation of modified nucleosides or by direct aqueous cross-coupling reactions of halogenated dNTPs with alkynes, arylboronic acids, or alkenes. The methods of polymerase synthesis include primer extension, PCR, nicking enzyme amplifications, and other methods which enable the synthesis of diverse types of long or short and double-stranded DNA or single-stranded oligonucleotides. The applications include labeling in diagnostics (labeling or coding of DNA bases) and chemical biology (bioconjugations, modulation of protein binding, etc.). Workplace Institute of Organic Chemistry and Biochemistry Contact asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Jana Procházková, Tel.: 220 183 418 Year of Publishing 2017
Number of the records: 1