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Manumycin A downregulates release of proinflammatory cytokines from TNF alpha stimulated human monocytes
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SYSNO ASEP 0460046 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Manumycin A downregulates release of proinflammatory cytokines from TNF alpha stimulated human monocytes Author(s) Cecrdlová, E. (CZ)
Petříčková, Kateřina (MBU-M) RID
Kolesár, L. (CZ)
Petříček, Miroslav (MBU-M) RID
Sekerková, A. (CZ)
Šváchová, V. (CZ)
Stříž, I. (CZ)Source Title Immunology Letters. - : Elsevier - ISSN 0165-2478
Roč. 169, JAN 2016 (2016), s. 8-14Number of pages 7 s. Language eng - English Country NL - Netherlands Keywords Manumycin A ; Cytokines ; Immunomodulation Subject RIV EC - Immunology R&D Projects LH12191 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) NT13012 GA MZd - Ministry of Health (MZ) Institutional support MBU-M - RVO:61388971 UT WOS 000369205200002 EID SCOPUS 84947903541 DOI 10.1016/j.imlet.2015.11.010 Annotation Macrolide antibiotics such as azithromycin or clarithromycin are known to have potent anti-inflammatory and immunomodulatory effects but these properties cannot be widely used due to a risk of bacterial resistance. We studied another polyketide antibiotic, structurally related manumycin A known as a streptomycete derived farnesyltransferase inhibitor with limited antibacterial effects, with respect to its potential regulation of mRNA expression of several genes associated with proinflammatory responses. Downregulation of mRNA for IL-6, TLR-8, IL-1 beta and IL-10 was found in THP-1 cells after 4 h stimulation with TNF alpha in the presence of manumycin A and downregulated TLR-8 and EGR-1 genes were observed after 8 h. Among the genes upregulated in response to manumycin were HMOX-1, TNFRSF10A, IL-1R1, TICAM2, NLRP12 after 4 h and only IL-1R1 after 8 h. Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2017
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