Efficient condensation of DNA into environmentally responsive polyplexes produced from block catiomers carrying amine or diamine groups

0456133 - ÚMCH 2017 RIV US eng J - Journal Article
Albuquerque, L. J. C. - Annes, K. - Milazzotto, M. P. - Mattei, B. - Riske, K. A. - Jäger, Eliezer - Pánek, Jiří - Štěpánek, Petr - Kapusta, Peter - Muraro, P. I. R. - De Freitas, A. G. O. - Schmidt, V. - Giacomelli, C. - Bonvent, J.-J. - Giacomelli, F. C.
Efficient condensation of DNA into environmentally responsive polyplexes produced from block catiomers carrying amine or diamine groups.
Langmuir. Roč. 32, č. 2 (2016), s. 577-586. ISSN 0743-7463
R&D Projects: GA MŠMT(CZ) LH14292
Institutional support: RVO:61389013 ; RVO:61388955
Keywords : polyplexes * DNA condensation * fluorescence
Subject RIV: CC - Organic Chemistry; CF - Physical ; Theoretical Chemistry (UFCH-W)
Impact factor: 3.833, year: 2016

The intracellular delivery of nucleic acids requires a vector system as they cannot diffuse across lipid membranes. Although polymeric transfecting agents have been extensively investigated, none of the proposed gene delivery vehicles fulfill all of the requirements needed for an effective therapy, namely, the ability to bind and compact DNA into polyplexes, stability in the serum environment, endosome-disrupting capacity, efficient intracellular DNA release, and low toxicity. The challenges are mainly attributed to conflicting properties such as stability vs efficient DNA release and toxicity vs efficient endosome-disrupting capacity. Accordingly, investigations aimed at safe and efficient therapies are still essential to achieving gene therapy clinical success.
Permanent Link: http://hdl.handle.net/11104/0256720