Influence of Culture Media on Microbial Fingerprints Using Raman Spectroscopy

0451404 - ÚPT 2016 RIV CH eng J - Journal Article
Mlynariková, K. - Samek, Ota - Bernatová, Silvie - Růžička, F. - Ježek, Jan - Hároniková, A. - Šiler, Martin - Zemánek, Pavel - Holá, V.
Influence of Culture Media on Microbial Fingerprints Using Raman Spectroscopy.
Sensors. Roč. 15, č. 11 (2015), s. 29635-29647. E-ISSN 1424-8220
R&D Projects: GA MŠMT ED0017/01/01; GA ČR(CZ) GA15-20645S; GA MŠMT(CZ) LO1212
Institutional support: RVO:68081731
Keywords : Raman spectroscopy * bacteria * yeasts * culture media
Subject RIV: BH - Optics, Masers, Lasers
Impact factor: 2.033, year: 2015

Raman spectroscopy has a broad range of applications across numerous scientific fields, including microbiology. Our work here monitors the influence of culture media on the Raman spectra of clinically important microorganisms (Escherichia coli, Staphylococcus aureus, Staphylococcus epidermidis and Candida albicans). Choosing an adequate medium may enhance the reproducibility of the method as well as simplifying the data processing and the evaluation. We tested four different media per organism depending on the nutritional requirements and clinical usage directly on a Petri dish. Some of the media have a significant influence on the microbial fingerprint (Roosvelt-Park Institute Medium, CHROMagar) and should not be used for the acquisition of Raman spectra. It was found that the most suitable medium for microbiological experiments regarding these organisms was Mueller-Hinton agar.
Permanent Link: http://hdl.handle.net/11104/0252574