Iron(II) Supramolecular Helicates Condense Plasmid DNA and Inhibit Vital DNA-Related Enzymatic Activities

0447649 - BFÚ 2016 RIV DE eng J - Journal Article
Malina, Jaroslav - Hannon, M.J. - Brabec, Viktor
Iron(II) Supramolecular Helicates Condense Plasmid DNA and Inhibit Vital DNA-Related Enzymatic Activities.
Chemistry - A European Journal. Roč. 21, č. 31 (2015), s. 11189-11195. ISSN 0947-6539. E-ISSN 1521-3765
R&D Projects: GA ČR(CZ) GA13-08273S
Institutional support: RVO:68081707
Keywords : TOPOISOMERASE-I * METALLOSUPRAMOLECULAR CYLINDERS * TRANSCRIPTIONAL ACTIVITY
Subject RIV: BO - Biophysics
Impact factor: 5.771, year: 2015

The dinuclear iron(II) supramolecular helicates [Fe2L3]Cl-4 (L= C25H20N4) bind to DNA through noncovalent (i. e., hydrogen-bonding, electrostatic) interactions and exhibit antimicrobial and anticancer effects. In this study, we show that the helicates condense plasmid DNA with a much higher potency than conventional DNA-condensing agents. Notably, molecules of DNA in the presence of the M enantiomer of [Fe2L]Cl-4 do not form intermolecular aggregates typically formed by other condensing agents, such as spermidine or spermine. The helicates inhibit the activity of several DNA-processing enzymes, such as RNA polymerase, DNA topoisomerase I, deoxyribonuclease I, and site-specific restriction endonucleases. However, the results also indicate that the DNA condensation induced by the helicates does not play a crucial role in these inhibition reactions. The mechanisms for the inhibitory effects of [Fe2L3]Cl-4 helicates on DNA-related enzymatic activities have been proposed.
Permanent Link: http://hdl.handle.net/11104/0249456