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A simple microviscometric approach based on Brownian motion tracking
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SYSNO ASEP 0446344 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title A simple microviscometric approach based on Brownian motion tracking Author(s) Hnyluchová, Z. (CZ)
Bjalončikova, P. (CZ)
Karas, P. (CZ)
Mravec, F. (CZ)
Halasová, T. (CZ)
Pekar, M. (CZ)
Kubala, Lukáš (BFU-R) RID, ORCID
Víteček, Jan (BFU-R) RID, ORCIDNumber of authors 8 Source Title Review of Scientific Instruments. - : AIP Publishing - ISSN 0034-6748
Roč. 86, č. 2 (2015)Number of pages 10 Publication form Online - E Language eng - English Country US - United States Keywords MULTIPLE-PARTICLE TRACKING ; MICRORHEOLOGY ; IMAGE Subject RIV BO - Biophysics R&D Projects GCP305/12/J038 GA ČR - Czech Science Foundation (CSF) Institutional support BFU-R - RVO:68081707 UT WOS 000350552700039 DOI 10.1063/1.4913386 Annotation Viscosity-an integral property of a liquid-is traditionally determined by mechanical instruments. The most pronounced disadvantage of such an approach is the requirement of a large sample volume, which poses a serious obstacle, particularly in biology and biophysics when working with limited samples. Scaling down the required volume by means of microviscometry based on tracking the Brownian motion of particles can provide a reasonable alternative. In this paper, we report a simple microviscometric approach which can be conducted with common laboratory equipment. The core of this approach consists in a freely available standalone script to process particle trajectory data based on a Newtonian model. In our study, this setup allowed the sample to be scaled down to 10 mu l. The utility of the approach was demonstrated using model solutions of glycerine, hyaluronate, and mouse blood plasma. Therefore, this microviscometric approach based on a newly developed freely available script can be suggested for determination of the viscosity of small biological samples (e.g., body fluids). (C) 2015 AIP Publishing LLC. Workplace Institute of Biophysics Contact Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Year of Publishing 2016
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