Zygosaccharomyces rouxii Trk1 is an efficient potassium transporter providing yeast cells with high lithium tolerance

0445013 - FGÚ 2016 RIV GB eng J - Journal Article
Zimmermannová, Olga - Salazar, A. - Sychrová, Hana - Ramos, J.
Zygosaccharomyces rouxii Trk1 is an efficient potassium transporter providing yeast cells with high lithium tolerance.
FEMS Yeast Research. Roč. 15, č. 4 (2015), fov029. ISSN 1567-1356. E-ISSN 1567-1364
R&D Projects: GA ČR(CZ) GAP503/10/0307
Institutional support: RVO:67985823
Keywords : Zygosaccharomyces rouxii * potassium transport * lithium tolerance * membrane potential * intracellular pH * pHluorin integration
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 2.479, year: 2015

Zygosaccharomyces rouxii is an osmotolerant yeast and is endowed with only one typical potassium transporter (ZrTrk1). We characterized ZrTrk1 activity and its contribution to various physiological parameters in detail. Our results show that ZrTrk1 is a high-affinity K+ transporting system efficiently discriminating between K+ and Li+. Upon ZrTrk1 heterologous expression in S. cerevisiae, it confers cells with a remarkably high lithium tolerance due to preventing Li+ influx into cells, it is able to complement a plasma-membrane hyperpolarization and a decrease in intracellular pH caused by the lack of endogenous K+ transporting systems. Our data corroborate a tight connection between potassium and proton transporters in yeasts and provides new insights into Z. rouxii cation homeostasis and the basis of its high osmotolerance
Permanent Link: http://hdl.handle.net/11104/0247457