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Two-Step Separation of Nostotrebin 6 from Cultivated Soil Cyanobacterium (Nostoc sp.) by High Performance Countercurrent Chromatography
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SYSNO ASEP 0440741 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Two-Step Separation of Nostotrebin 6 from Cultivated Soil Cyanobacterium (Nostoc sp.) by High Performance Countercurrent Chromatography Author(s) Cheel, José (MBU-M) RID, ORCID
Kučerová, P. (CZ)
Garrard, I. (GB)
Ignatova, S. (GB)
Hrouzek, Pavel (MBU-M) ORCID
Kopecký, Jiří (MBU-M) ORCIDNumber of authors 6 Source Title Molecules. - : MDPI
Roč. 19, č. 4 (2014), s. 8773-8787Number of pages 15 s. Language eng - English Country CH - Switzerland Keywords nostotrebin 6 ; cyanobacterium ; Nostoc ; HPLC separation Subject RIV EE - Microbiology, Virology R&D Projects ED2.1.00/03.0110 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) EE2.3.30.0059 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support MBU-M - RVO:61388971 UT WOS 000340036200006 Annotation High performance countercurrent chromatography (HPCCC) was successfully applied for the separation of nostotrebin 6 from cultivated soil cyanobacteria in a two-step operation. A two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water (4:5:4:5, v/v/v/v) was employed for the HPCCC separation. In the first-step operation, its neutral upper phase was used as stationary phase and its basic lower phase (1% NH3 in lower phase) was employed as mobile phase at a flow rate of 1 mL/min. In the second operation step, its neutral upper phase was used as stationary phase, whereas both its neutral lower phase and basic lower phase were employed as mobile phase with a linear gradient elution at a flow rate of 0.8 mL/min. The revolution speed and temperature of the separation column were 1,000 rpm and 30 degrees C, respectively. Using HPCCC followed by clean-up on Sephadex LH-20 gel, 4 mg of nostotrebin 6 with a purity of 99% as determined by HPLC/DAD-ESI-HRMS was obtained from 100 mg of crude extract. The chemical identity of the isolated compound was confirmed by comparing its spectroscopic data (UV, ESI-HRMS, ESI-HRMS2) with those of an authentic standard and data available in the literature. Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2015
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