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Capillary Electrophoresis of Carbohydrates: From Monosaccharides to Complex Plysaccharides
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SYSNO ASEP 0437343 Document Type M - Monograph Chapter R&D Document Type Monograph Chapter Title Extracellular Polysaccharides in Microbial Biofilm and Their Influence on the Electrophoretic Properties of Microbial Cells Author(s) Růžička, F. (CZ)
Horká, Marie (UIACH-O) RID, ORCID
Holá, V. (CZ)Number of authors 3 Source Title Capillary Electrophoresis of Carbohydrates: From Monosaccharides to Complex Plysaccharides. - Totowa : Humana Press Inc, 2011 / Volpi N. - ISBN 978-1-60761-874-4 Pages s. 105-126 Number of pages 22 s. Number of pages 277 Publication form Print - P Language eng - English Country US - United States Keywords capillary zone electrophoresis ; laser induced flurescence ; high performance electrophoresis Subject RIV CB - Analytical Chemistry, Separation Institutional support UIACH-O - RVO:68081715 UT WOS 000284073400005 DOI 10.1007/978-1-60761-875-1_5 Annotation The surfaces of biofilm-positive microorganisms are usually covered with biofilm-specific extracellular polysaccharide substances that play a key role in a biofilm formation and function [1,2] The presence of this substance on the surface can affect the physicochemical properties of the bacterial cell, including the cell-surface hydrophobicity and surface charge The differences in the surface charges lead to the different isoelectric points and the different electromigration characteristics of biofilm-positive and biofilm-negative cells For this reason, the use of the capillary electrophoresis, especially capillary isoelectric focusing (CIEF) and capillary zone electrophoresis (CZE) [3,4], appears to be the appropriate method for the detection of biofilm positivity in a particular strain, as well as for the separation of biofilm-positive and biofilm-negative strains. Workplace Institute of Analytical Chemistry Contact Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234 Year of Publishing 2015
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