Number of the records: 1
Membrane activity of the pentaene macrolide didehydroroflamycoin in 2 model lipid bilayers
- 1.
SYSNO ASEP 0436047 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Membrane activity of the pentaene macrolide didehydroroflamycoin in 2 model lipid bilayers Author(s) Koukalová, Alena (UFCH-W) RID
Pokorná, Šárka (UFCH-W) RID
Fišer, Radovan (MBU-M) RID, ORCID
Kopecký, Jr., V. (CZ)
Humpolíčková, Jana (UFCH-W) RID
Černý, J. (CZ)
Hof, Martin (UFCH-W) RID, ORCIDSource Title Biochimica Et Biophysica Acta-Biomembranes. - : Elsevier - ISSN 0005-2736
Roč. 1848, č. 2 (2015), s. 444-452Number of pages 9 s. Language eng - English Country NL - Netherlands Keywords Didehydroroflamycoin ; Filipin III ; Amphotericin B Subject RIV CF - Physical ; Theoretical Chemistry R&D Projects GC14-03141J GA ČR - Czech Science Foundation (CSF) LH13259 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UFCH-W - RVO:61388955 ; MBU-M - RVO:61388971 UT WOS 000348687500008 EID SCOPUS 84912061294 DOI 10.1016/j.bbamem.2014.10.038 Annotation Didehydroroflamycoin (DDHR), a recently isolatedmember of the polyenemacrolide family, was shown to have 21 antibacterial and antifungal activity. However, its mechanism of action has not been investigated. Antibiotics 22 from this family are amphiphilic; thus, they have membrane activity, their biological action is localized in the 23 membrane, and the membrane composition and physical properties facilitate the recognition of a particular 24 compound by the target organism. In this work, we use model lipid membranes comprised of giant unilamellar 25 vesicles (GUVs) for a systematic study of the action of DDHR. In parallel, experiments are conducted using filipin 26 III and amphotericin B, other members of the family, and the behavior observed for DDHR is described in the 27 context of that of these two heavily studied compounds. The study shows that DDHR disrupts membranes via 28 two different mechanisms and that the involvement of these mechanisms depends on the presence of 29 cholesterol. The leakage assays performed in GUVs and the conductance measurements using black lipid 30 membranes (BLM) reveal that the pores that develop in the absence of cholesterol are transient and their size 31 is dependent on the DDHR concentration. In contrast, cholesterol promotes the formation of more defined 32 structures that are temporally stable. Workplace J. Heyrovsky Institute of Physical Chemistry Contact Michaela Knapová, michaela.knapova@jh-inst.cas.cz, Tel.: 266 053 196 Year of Publishing 2016
Number of the records: 1