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Microarray analysis of serum mRNA in patients with head and neck squamous cell carcinoma at whole-genome scale
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SYSNO ASEP 0435343 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Microarray analysis of serum mRNA in patients with head and neck squamous cell carcinoma at whole-genome scale Author(s) Čapková, M. (CZ)
Šáchová, Jana (UMG-J)
Strnad, Hynek (UMG-J) RID
Kolář, Michal (UMG-J) RID, ORCID
Hroudová, Miluše (UMG-J) RID
Chovanec, M. (CZ)
Čada, Z. (CZ)
Štefl, M. (CZ)
Valach, J. (CZ)
Kastner, J. (CZ)
Smetana, K. Jr. (CZ)
Plzák, J. (CZ)Source Title BioMed Research International. - : Hindawi - ISSN 2314-6133
-, April 23 (2014)Number of pages 10 s. Language eng - English Country US - United States Keywords Microarray Analysis ; Head and Neck Squamous Cell Carcinoma ; whole-genome scale Subject RIV EB - Genetics ; Molecular Biology R&D Projects NT13488 GA MZd - Ministry of Health (MZ) Institutional support UMG-J - RVO:68378050 UT WOS 000335235800001 DOI 10.1155/2014/408683 Annotation With the increasing demand for noninvasive approaches in monitoring head and neck cancer, circulating nucleic acids have been shown to be a promising tool. We focused on the global transcriptome of serum samples of head and neck squamous cell carcinoma (HNSCC) patients in comparison with healthy individuals. We compared gene expression patterns of 36 samples. Twenty-four participants including 16 HNSCC patients (from 12 patients we obtained blood samples 1 year posttreatment) and 8 control subjects were recruited. The Illumina HumanWG-6 v3 Expression BeadChip was used to profile and identify the differences in serum mRNA transcriptomes. We found 159 genes to be significantly changed (Storey's P value <0.05) between normal and cancer serum specimens regardless of factors including p53 and B-cell lymphoma family members (Bcl-2, Bcl-XL). In contrast, there was no difference in gene expression between samples obtained before and after surgery in cancer patients. We suggest that microarray analysis of serum cRNA in patients with HNSCC should be suitable for refinement of early stage diagnosis of disease that can be important for development of new personalized strategies in diagnosis and treatment of tumours but is not suitable for monitoring further development of disease. Workplace Institute of Molecular Genetics Contact Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Year of Publishing 2015
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