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Advances in Biomedical Spectroscopy
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SYSNO ASEP 0425093 Document Type M - Monograph Chapter R&D Document Type The record was not marked in the RIV Title Antimicrobial peptide from the eusocial bee Halictus sexcinctus interacting with model membranes Author(s) Pazderková, Markéta (UOCHB-X) RID, ORCID
Kočišová, E. (CZ)
Pazderka, T. (CZ)
Maloň, Petr (UOCHB-X)
Kopecký, V. Jr. (CZ)
Monincová, Lenka (UOCHB-X)
Čeřovský, Václav (UOCHB-X) RID, ORCID
Bednárová, Lucie (UOCHB-X) RID, ORCIDNumber of authors 8 Source Title Advances in Biomedical Spectroscopy. - Amsterdam : IOS Press, 2013 / Marques M. P. ; Batista de Carvalho L. A. E. ; Haris P. I. - ISSN 1875-0656 - ISBN 978-1-61499-183-0 Pages s. 79-83 Number of pages 5 s. Number of pages 316 Publication form Print - P Language eng - English Country NL - Netherlands Keywords antibacterial peptides ; circular dichroism ; fluorescence ; halictine ; infrared spectroscopy ; liposome ; micelle ; phospholipid Subject RIV CC - Organic Chemistry CEZ AV0Z40550506 - UOCHB-X (2005-2011) EID SCOPUS 84888093507 Annotation Halictine-1 (Hal-1)-a linear antibacterial dodecapeptide isolated from the venom of the eusocial bee Halictus sexcinctus-has been subjected to a detailed spectroscopic study including circular dichroism, fluorescence and vibrational spectroscopy. We investigated Hal-1's ability to adopt an amphipathic α-helical structure upon interaction with model lipid based bacterial membranes (phosphatidylcholine/phosphatidylglycerol based large unilamellar vesicles, sodium dodecylsulfate micelles) and helix inducing components (trifluoroethanol). It was found that Hal-1 responds sensitively to composition of the membrane model and to peptide/lipid ratio. Amphipathic nature of the helical Hal-1 seems to favour flat charged surfaces of the model lipid particles over the non-directional interaction with trifluoroethanol. Increasing fraction of polyproline II type conformation was detected at low peptide/lipid ratios. Workplace Institute of Organic Chemistry and Biochemistry Contact asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Jana Procházková, Tel.: 220 183 418 Year of Publishing 2014
Number of the records: 1