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Involvement of phosphatidylinositol 4,5-bisphosphate in RNA polymerase I transcription
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SYSNO ASEP 0423030 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Involvement of phosphatidylinositol 4,5-bisphosphate in RNA polymerase I transcription Author(s) Yildirim, Sukriye (UMG-J)
Castano, Enrique (UMG-J)
Sobol, Margaryta (UMG-J) RID
Philimonenko, Vlada (UMG-J)
Dzijak, Rastislav (UMG-J) RID
Venit, Tomáš (UMG-J) RID, ORCID
Hozák, Pavel (UMG-J) RID, ORCIDSource Title Journal of Cell Science. - : Company of Biologists - ISSN 0021-9533
Roč. 126, č. 12 (2013), s. 2730-2739Number of pages 10 s. Publication form Print - P Language eng - English Country GB - United Kingdom Keywords nucleolus ; transcription ; PIP2 ; UBF ; fibrillarin Subject RIV EB - Genetics ; Molecular Biology R&D Projects GAP305/11/2232 GA ČR - Czech Science Foundation (CSF) GD204/09/H084 GA ČR - Czech Science Foundation (CSF) LC545 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LC06063 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UMG-J - RVO:68378050 UT WOS 000320705300018 DOI 10.1242/jcs.123661 Annotation RNA polymerase I (Pol I) transcription is essential for the cell cycle, growth and protein synthesis in eukaryotes. In the present study, we found that phosphatidylinositol 4,5-bisphosphate (PIP2) is a part of the protein complex on the active ribosomal promoter during transcription. PIP2 makes a complex with Pol I and the Pol I transcription factor UBF in the nucleolus. PIP2 depletion reduces Pol I transcription, which can be rescued by the addition of exogenous PIP2. In addition, PIP2 also binds directly to the pre-rRNA processing factor fibrillarin (Fib), and co-localizes with nascent transcripts in the nucleolus. PIP2 binding to UBF and Fib modulates their binding to DNA and RNA, respectively. In conclusion, PIP2 interacts with a subset of Pol I transcription machinery, and promotes Pol I transcription. Workplace Institute of Molecular Genetics Contact Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Year of Publishing 2014
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