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Towards crystal structures of antibodies and transcription factors
- 1.0398525 - ÚOCHB 2014 RIV CZ eng J - Journal Article
Písačková, Jana - Procházková, Kateřina - Král, Vlastimil - Fábry, Milan - Řezáčová, Pavlína
Towards crystal structures of antibodies and transcription factors.
Materials Structure in Chemistry, Biology, Physics and Technology. Roč. 20, č. 2 (2013), s. 121-123. ISSN 1211-5894
R&D Projects: GA MŠMT ME08016; GA MŠMT 1M0505; GA ČR GA203/09/0820
Institutional research plan: CEZ:AV0Z40550506; CEZ:AV0Z50520514
Keywords : scFv antibody fragment * DeoR repressor protein * thermofluor assay * protein crystallization
Subject RIV: FR - Pharmacology ; Medidal Chemistry
Protein three-dimensional structure determination by X-ray crystallography is important for understanding protein function. The protein crystallization bottleneck can be overcome by optimization of factors which influence the protein crystallizability. We demonstrated this approach on two scFv antibody fragments, and on prokaryotic metabolic regulator. We have performed protein pre-crystallization analysis employing the combination of size-exclusion chromatography, dynamic light scattering, and thermofluor assay. Based on the analysis data, we have developed crystallization protocols which yielded monocrystals suitable for X-ray diffraction analysis. This enabled us to determine crystal structure of the effector-binding domain of DeoR repressor.
Permanent Link: http://hdl.handle.net/11104/0226021
Number of the records: 1