Number of the records: 1
Expression, function and regulation of Evi-1 during embryonic avian development
- 1.
SYSNO ASEP 0394981 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Expression, function and regulation of Evi-1 during embryonic avian development Author(s) Celá, Petra (UZFG-Y) RID
Moravcová Balková, Simona (UZFG-Y)
Bryjová, Anna (UBO-W) ORCID, RID, SAI
Horáková, D. (CZ)
Míšek, Ivan (UZFG-Y)
Richman, J. M. (CA)
Buchtová, Marcela (UZFG-Y) ORCID, RIDSource Title Gene Expression Patterns. - : Elsevier - ISSN 1567-133X
Roč. 13, č. 8 (2013), s. 343-353Number of pages 11 s. Publication form Print - P Language eng - English Country NL - Netherlands Keywords ecotropical viral integration site 1 ; chondrogenesis ; siRNA ; limb patterning Subject RIV EA - Cell Biology Subject RIV - cooperation Institute of Vertebrate Biology - Zoology R&D Projects GA304/09/0725 GA ČR - Czech Science Foundation (CSF) Institutional support UZFG-Y - RVO:67985904 ; UBO-W - RVO:68081766 UT WOS 000328595100008 EID SCOPUS 84880912706 DOI https://doi.org/10.1016/j.gep.2013.06.002 Annotation Ecotropical viral integration site 1 (Evi-1) is a transcription factor essential for vascularisation and cell proliferation during embryonic development. The chimeric transcription factor AML1-EVI-1 is activated in leukaemia where it plays a role as a differentiation block and stimulator of proliferation. Here, we cloned chicken Evi-1 and analysed its expression during embryonic development. There was early expression in the pharyngeal arches, in the brain and intermediate mesoderm of chicken embryos at stage 15. Later at stage 20, Evi-1 mesenchymal expression was concentrated in the second pharyngeal arch, and weaker expression was found in the mandibular and maxillary prominences. Facial expression decreased in intensity during development. Evi-1 expression in the limb was also limited to the mesenchyme with the most prominent expression in the anterior margin. Evi-1 was not detectable in the posterior limb bud. At later stages, Evi-1 was expressed in the peripheral mesenchyme of the limb but not in the developing precartilage blastema. At stage 29, the expression became restricted to the perichondrium and interdigital areas; however, the cartilage condensations themselves were negative. To study the function of Evi-1 in chondrogenesis, we knocked down expression in limb micromass cultures using siRNA. Chondrogenesis was significantly reduced in both anterior and posterior cultures. Since Evi-1 was expressed adjacent to the apical ectodermal ridge and this area is a source of FGFs, we tested whether endogenous FGF receptor signalling was necessary to maintain its expression. Inhibitors of FGFRs (PD161570 and SU5402) were applied to wing mesenchyme, and downregulation of Evi-1 expression was observed after treatment with both inhibitors. Therefore, Evi-1 may be a transcription factor mediating the effects of FGF and may also be defining the size of cartilage elements in the limb. Workplace Institute of Animal Physiology and Genetics Contact Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Year of Publishing 2014
Number of the records: 1