Dependence of spontaneous electrical activity and basal prolactin release on nonselective cation channels in pituitary lactotrophs

0385667 - FGÚ 2013 RIV CZ eng J - Journal Article
Kučka, M. - Kretschmannová, K. - Stojilkovic, S. S. - Zemková, Hana - Tomic, M.
Dependence of spontaneous electrical activity and basal prolactin release on nonselective cation channels in pituitary lactotrophs.
Physiological Research. Roč. 61, č. 3 (2012), s. 267-275. ISSN 0862-8408. E-ISSN 1802-9973
R&D Projects: GA ČR(CZ) GA305/07/0681; GA ČR(CZ) GBP304/12/G069
Institutional research plan: CEZ:AV0Z50110509
Institutional support: RVO:67985823
Keywords : TRPC channels * pituitary * GH3 cells * calcium influx * action potentials * resting membrane potential
Subject RIV: ED - Physiology
Impact factor: 1.531, year: 2012

In cultured anterior pituitary cells, including lactotrophs and immortalized GH3 cells, replacement of extracellular Na+ with large organic cations led to an instantaneous hyperpolarization of cell membranes that was associated with a cessation of spontaneous firing. Selective blockers of TRPC channels, inhibited electrical activity, Ca2+ influx and prolactin release in a concentration-dependent manner. Quantitative RT-PCR analysis revealed the high expression of mRNA transcripts for TRPC1 in both lactotrophs and GH3 cells. These results indicate that nonselective cation channels, presumably belonging to the TRPC family, contribute to the background depolarizing conductance and firing of action potentials with consequent contribution to Ca2+ influx and hormone release in lactotrophs and GH3 cells
Permanent Link: http://hdl.handle.net/11104/0214784