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Plant Aurora kinases play a role in maintenance of primary meristems and control of endoreduplication
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SYSNO ASEP 0380510 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Plant Aurora kinases play a role in maintenance of primary meristems and control of endoreduplication Author(s) Petrovská, Beáta (UEB-Q) RID, ORCID
Cenklová, Věra (UEB-Q)
Pochylová, Žaneta (UEB-Q)
Kourová, Hana (MBU-M)
Doskočilová, Anna (MBU-M)
Plíhal, Ondřej (MBU-M)
Binarová, Lenka (UEB-Q)
Binarová, Pavla (MBU-M) RID, ORCIDSource Title New Phytologist - ISSN 0028-646X
Roč. 193, č. 3 (2012), s. 590-604Number of pages 15 s. Language eng - English Country GB - United Kingdom Keywords Arabidopsis thaliana ; AtTPX2 (targeting protein for Xklp2) ; Aurora kinases Subject RIV EB - Genetics ; Molecular Biology R&D Projects GA204/07/1169 GA ČR - Czech Science Foundation (CSF) GP204/09/P155 GA ČR - Czech Science Foundation (CSF) GD204/09/H084 GA ČR - Czech Science Foundation (CSF) LC06034 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LC545 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) IAA500200719 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) CEZ AV0Z50380511 - UEB-Q (2005-2011) AV0Z50200510 - MBU-M (2005-2011) UT WOS 000298984900006 DOI 10.1111/j.1469-8137.2011.03989.x Annotation The conserved family of Aurora kinases has multiple functions during mitosis. The roles of plant Aurora kinases have been characterized using inhibitor treatments. We down-regulated Aurora kinases in Arabidopsis thaliana using RNA interference (RNAi). We carried out a detailed phenotypic analysis of Aurora RNAi plants, biochemical and microscopic studies of AtAurora1 kinase together with AtTPX2 (targeting protein for Xklp2) and gama-tubulin. Cell division defects were observed in plants with reduced expression of Aurora kinases. Furthermore, the maintenance of primary meristems was compromised and RNAi seedlings entered endoreduplication prematurely. AtAurora1, its activator AtTPX2, and gama-tubulin were associated with microtubules in vitro; they were attached to regrowing kinetochore microtubules and colocalized on spindle microtubules and with a subset of early phragmoplast microtubules. Only the AtAurora1 kinase was translocated to the area of the cell plate. RNAi silencing of Aurora kinases showed that, in addition to their function in regulating mitosis, the kinases are required for maintaining meristematic activity and controlling the switch from meristematic cell proliferation to differentiation and endoreduplication. The colocalization and co-fractionation of AtAurora1 with AtTPX2, and gama-tubulin on microtubules in a cell cycle-specific manner suggests that AtAurora1 kinase may function to phosphorylate substrates that are critical to the spatiotemporal regulation of acentrosomal microtubule formation and organization. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2013
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