Isolation of antimicrobial peptides and proteins from tomato

0370591 - ÚOCHB 2012 CZ eng C - Conference Paper (international conference)
Králová, M. - Šanda, Miloslav - Macková, M. - Macek, Tomáš
Isolation of antimicrobial peptides and proteins from tomato.
Biologically Active Peptides. 12th Conference. Praha: Institute of Organic Chemistry and Biochemistry AS CR, v. v. i, 2011 - (Slaninová, J.), s. 73-76. Collection Symposium Series, 13. ISBN 978-80-86241-44-9.
[Biologically Active Peptides /12./. Praha (CZ), 27.04.2011-29.04.2011]
Grant - others:GA ČR(CZ) GA522/09/1693; GA ČR(CZ) GD305/09/H008
Program: GA; GD
Institutional research plan: CEZ:AV0Z40550506
Keywords : tomato * antimicrobial peptides * plant peptide isolation
Subject RIV: CC - Organic Chemistry

Recent emerging problem is occurrence of multiresistant pathogens. This inconvenience makes people to search for new antimicrobials. One of possible ways is looking for natural antimicrobial peptides. In our project, green tissues of tomato (Solanum lycopersicum) were chosen for isolation of such peptides. Extracts obtained were fractionated by solid phase extraction and liquid chromatography. Antimicrobial activities were tested on model microorganisms. Several fractions exhibited antimicrobial activity against bacteria and fungi. In the extracts, precursors of two antifungal proteins were identified.
Permanent Link: http://hdl.handle.net/11104/0204342