Enzymatic synthesis of dimeric glycomimetic ligands of NK cell activation receptors

Drozdová, Anna; Bojarová, Pavla; Křenek, Karel; Weignerová, Lenka; Hensen, B.; Elling, L.; Christensen, H.; Jensen, H.H.; Pelantová, Helena; Kuzma, Marek; Bezouška, Karel; Krupová, Monika; Adámek, David; Slámová, Kristýna; Křen, Vladimír

doi:https://dx.doi.org/10.1016/j.carres.2011.04.043

Number of the records: 1

Enzymatic synthesis of dimeric glycomimetic ligands of NK cell activation receptors

1.

SYSNO ASEP	0366522
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Enzymatic synthesis of dimeric glycomimetic ligands of NK cell activation receptors
Author(s)	Drozdová, Anna (MBU-M) Bojarová, Pavla (MBU-M)_ORCID Křenek, Karel (MBU-M) Weignerová, Lenka (MBU-M)_RID Hensen, B. (DE) Elling, L. (DE) Christensen, H. (DK) Jensen, H.H. (DK) Pelantová, Helena (MBU-M)_{ORCID, RID} Kuzma, Marek (MBU-M)_{ORCID, RID} Bezouška, Karel (MBU-M) Krupová, Monika (MBU-M) Adámek, David (MBU-M) Slámová, Kristýna (MBU-M)_{RID, ORCID} Křen, Vladimír (MBU-M)_{RID, ORCID}
Source Title	Carbohydrate Research. - : Elsevier - ISSN 0008-6215 Roč. 346, č. 12 (2011), s. 1599-1609
Number of pages	11 s.
Language	eng - English
Country	NL - Netherlands
Keywords	beta-N-Acetylhexosaminidase ; alactosyltransferase ; Enzymatic glycosylation
Subject RIV	CE - Biochemistry
R&D Projects	GP203/09/P024 GA ČR - Czech Science Foundation (CSF)
	GD305/09/H008 GA ČR - Czech Science Foundation (CSF)
	GA303/09/0477 GA ČR - Czech Science Foundation (CSF)
CEZ	AV0Z50200510 - MBU-M (2005-2011)
UT WOS	000292852100029
DOI	10.1016/j.carres.2011.04.043
Annotation	This work reveals new structural relationships in the complex process of the interaction between activation receptors of natural killer cells (rat NKR-P1, human CD69) and novel bivalent carbohydrate glycomimetics. The length, glycosylation pattern and linker structure of receptor ligands were examined with respect to their ability to precipitate the receptor protein from solution, which simulates the in vivo process of receptor aggregation during NK cell activation. It was found that di-LacdiNAc triazole compounds show optimal performance, reaching up to 100 percent precipitation of the present protein receptors, and achieving high immunostimulatory activities without any tendency to trigger activation-induced apoptosis. In the synthesis of the compounds tested, two enzymatic approaches were applied
Workplace	Institute of Microbiology
Contact	Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
Year of Publishing	2012

Number of the records: 1