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Redox labelling of nucleic acids for analyzing nucleotide sequences and monitoring DNA-protein interactions
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SYSNO ASEP 0361621 Document Type C - Proceedings Paper (int. conf.) R&D Document Type The record was not marked in the RIV Title Redox labelling of nucleic acids for analyzing nucleotide sequences and monitoring DNA-protein interactions Author(s) Fojta, Miroslav (BFU-R) RID, ORCID
Havran, Luděk (BFU-R) RID, ORCID
Horáková Brázdilová, Petra (BFU-R)
Pivoňková, Hana (BFU-R) ORCID
Kostečka, Pavel (BFU-R)
Macíčková-Cahová, Hana (UOCHB-X) RID, ORCID
Raindlová, Veronika (UOCHB-X) RID
Vrábel, Milan (UOCHB-X) RID, ORCID
Hocek, Michal (UOCHB-X) RID, ORCIDNumber of authors 9 Source Title Chemistry of Nucleic Acid Components. 15th Symposium. - Praha : Institute of Organic Chemistry and Biochemistry AS CR, v. v. i., 2011 / Hocek M. - ISBN 978-80-86241-37-1 Pages s. 155-158 Number of pages 4 s. Action Chemistry of Nucleic Acid Components /15./ Event date 05.06.2011-10.06.2011 VEvent location Český Krumlov Country CZ - Czech Republic Event type EUR Language eng - English Country CZ - Czech Republic Keywords DNA ; electrochemistry ; redox labeling Subject RIV CC - Organic Chemistry R&D Projects LC512 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LC06035 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) IAA400040901 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) IAA400040903 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) GA203/09/0317 GA ČR - Czech Science Foundation (CSF) GPP206/11/P739 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) CEZ AV0Z40550506 - UOCHB-X (2005-2011) AV0Z50040507 - BFU-R (2005-2011) AV0Z50040702 - BFU-R (2007-2013) Annotation Nucleobase labelling of DNA for electrochemical sensing was attained through chemical modification of thymine bases with osmium tetroxide in the presence of nitrogenous ligands, or via enzymatic incorporation of nucleotide conjugates with redox-active moieties using labelled deoxynucleoside triphosphates. DNA hybridization, primer extension and PCR techniques were used for sequence-specific DNA assays. Tail-labelled DNA substrates were applied to monitor DNA binding by tumour suppressor p53 protein. Workplace Institute of Organic Chemistry and Biochemistry Contact asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Jana Procházková, Tel.: 220 183 418 Year of Publishing 2012
Number of the records: 1