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Raman spectroscopy for the characterization of algal cells
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SYSNO ASEP 0352210 Document Type C - Proceedings Paper (int. conf.) R&D Document Type Conference Paper Title Raman spectroscopy for the characterization of algal cells Author(s) Samek, Ota (UPT-D) RID, ORCID, SAI
Jonáš, Alexandr (UPT-D) RID, SAI, ORCID
Pilát, Zdeněk (UPT-D) RID, SAI, ORCID
Zemánek, Pavel (UPT-D) RID, SAI, ORCID
Nedbal, Ladislav (UEK-B)
Tříska, Jan (UEK-B) RID, ORCID, SAI
Kotas, Petr (UEK-B) RID
Trtílek, M. (CZ)Number of authors 8 Source Title 17th Slovak-Czech-Polish Optical Conference on Wave and Quantum Aspects of Contemporary Optics (Proceedings of SPIE Vol. 7746). - Bellingham : SPIE, 2010 - ISBN 978-0-8194-8236-5 Pages 77460x: 1-7 Number of pages 7 s. Action Slovak-Czech-Polish Optical Conference on Wave and Quantum Aspects of Contemporary Optics /17./ Event date 06.09.2010 VEvent location Liptovsky Jan Country SK - Slovakia Event type WRD Language eng - English Country US - United States Keywords Raman spectroscopy ; algal cells ; lipids ; iodine value Subject RIV BH - Optics, Masers, Lasers R&D Projects ED0017/01/01 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) OC08034 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z20650511 - UPT-D (2005-2011) AV0Z60870520 - UEK-B (2005-2011) UT WOS 000291389400033 Annotation Raman spectroscopy can elucidate fundamental questions about intercellular variability and what governs it. Moreover, knowing the metabolic response on single cell level this can significantly contribute to the study and use of microalgae in systems biology and biofuel technology. Raman spectroscopy is capable to measure nutrient dynamics and metabolism in vivo, in real-time, label free making it possible to monitor/evaluate population variability. Also, degree of unsaturation of the algae oil (iodine value) can be measured using Raman spectra obtained from single microalgae. The iodine value is the determination of the amount of unsaturation contained in fatty acids (in the form of double bonds). Here we demonstrate the capacity of the spatially resolved Raman microspectroscopy to determine the effective iodine value in lipid storage bodies of individual living algal cells. Workplace Institute of Scientific Instruments Contact Martina Šillerová, sillerova@ISIBrno.Cz, Tel.: 541 514 178 Year of Publishing 2011
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