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A method for efficient extraction of rotifers (Rotifera) from soils
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SYSNO ASEP 0347122 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title A method for efficient extraction of rotifers (Rotifera) from soils Author(s) Devetter, Miloslav (BC-A) RID, ORCID Source Title Pedobiologia. - : Elsevier - ISSN 0031-4056
Roč. 53, č. 2 (2010), s. 115-118Number of pages 4 s. Language eng - English Country DE - Germany Keywords soil rotifers ; Bdelloids ; quantitative extraction Subject RIV EH - Ecology, Behaviour R&D Projects GP206/06/P405 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z60660521 - UPB-H, BC-A (2005-2011) UT WOS 000275286900002 DOI 10.1016/j.pedobi.2009.07.001 Annotation Rotifers are a group of soil animals which are not commonly quantified. The most recent data originated from soil studies focussed on nematodes and various modifications of the Baermann funnel, which is not suitable for rotifers. The present study developed a method for quantitative extraction of rotifers, which gives representative results describing rotifer populations and facilitates subsequent identification to the species level. The LC extractor recovered rotifer numbers that were two orders of magnitude higher than what was extracted from the Baermann funnel. The method involves spreading a soil sample on a plastic sieve and uses a very thin layer of cellulose submerged in distilled water in a Petri dish. The Petri dish is situated on a cooled panel and light from the top by fluorescent lamps. The temperature of the base panel which gave the best extraction resutls was determined experimentally to be 5°C. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2011
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